pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMU10.34215/1609-1175-2022-1-46-55pmj-2299Research ArticleОБЗОРЫREVIEWSВирусные векторы в трансгенных исследованиях: перспективы использования для лечения болезней ЦНС и генной терапииViral Vectors in Transgenic Research: Prospects for the Treatment of CNS Diseases and Gene Therapyhttps://orcid.org/0000-0003-0388-3147ПущинаЕ. В.PushchinaE. V.

Пущина Евгения Владиславовна – доктор биологических наук, профессор РАН, главный научный сотрудник лаборатории Клеточной дифференциации.

690041, Владивосток, ул. Пальчевского, 17; тел.: +79990400751

Pushchina Evgeniya Vladislavovna – Doctor biol. Sciences, Professor of the Russian Academy of Sciences, Chief Researcher of the Laboratory of Cellular Differentiation.

690041, Vladivostok, Palchevsky St., 17; tel.: +79990400751

puschina@mail.ruКапустяновИ. А.KapustyanovI. A.

Капустянов Илья Александрович - аспирант лаборатории Клеточной дифференциации.

690041, Владивосток, ул. Пальчевского, 17, тел.: +79020521267

Ilya A. Kapustyanov - Post-graduate student of the Laboratory of Cellular Differentiation.

690041, Vladivostok, Palchevsky St., 17, tel.: +79020521267

ilyaak9772@gmail.comВараксинА. А.VaraksinA. A.

Вараксин Анатолий Алексеевич - доктор биологических наук, профессор, ведущий научный сотрудник лаборатории Клеточной дифференциации

690041, Владивосток, ул. Пальчевского, 17, тел.: +79146723560

Varaksin Anatoly Alekseevich - Doctor biol. Sciences, Professor, Leading Researcher, Laboratory of Cellular Differentiation

690041, Vladivostok, Palchevsky St., 17, tel.: +79146723560

anvaraksin@mail.ruНациональный научный центр морской биологии им. А.В. Жирмунского, Дальневосточное отделение Российской академииРоссияA.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of SciencesRussian Federation202226032022014655Copyright © Пущина Е.В., Капустянов И.А., Вараксин А.А., 20222022Пущина Е.В., Капустянов И.А., Вараксин А.А.Pushchina E.V., Kapustyanov I.A., Varaksin A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/2299

Вирусные переносчики представляют большой клинический интерес из-за их высокой эффективности, позволяющей находить практическое применение в генной терапии. В обзоре приводятся современные данные исследований на различных видах рыб в качестве потенциальных моделей для использования вирусных векторов. В ходе изучения высокопроизводительных аденовирусных векторов получены данные об устойчивой экспрессии трансгенов в организме Danio rerio и других видов рыб. Рассмотрены особенности применения аденоассоциированных векторов в исследовании транснейронального переноса белка глюкоронидазы в проекционных нейронах вентральной тегментальной области и стриатума для лечения метаболической недостаточности при мукополисахаридозе VII. Принимая во внимание широкий репертуар генетически модифицированных линий Danio rerio, этические аспекты и допустимость этой модели в нейрогеннных исследованиях, очевидно, что Danio rerio, как и другие виды рыб, могут быть альтернативой для ранней стадии доклинической оценки эффективности вирусных векторов.

Viral vectors are of great clinical interest due to their high efficiency, which allows them to find practical applications in gene therapy. The review presents current research data on various fish species as potential models for the use of viral vectors. During the study of high-performance adenoviral vectors data on the stable expression of transgenes in the body of Danio rerio and other fish species was obtained. The features of the use of adeno-associated vectors in the study of transneuronal transfer of the glucuronidase protein in the projection neurons of the ventral tegmental area and striatum to treat metabolic insufficiency in case of mucopolysaccharidosis VII are considered. Taking into consideration the wide set of genetically modified Danio rerio strains, ethical aspects, and the acceptability of applying this model in neurogenic studies, it is clear that Danio rerio, as well as other fish species, can be used as an alternative for early preclinical evaluation of the efficiency of viral vectors.

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The authors declare that there are no conflicts of interest present.