pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMU10.34215/1609-1175-2023-1-38-43pmj-2468Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESСистема для изучения механизмов флокуляции и автоагрегации бактерий, основанная на магнитной левитацииA magnetic levitation-based system to study the mechanisms of bacterial flocculation and autoaggregationДомнинП. А.DomninP. A.

Москва

Moscow

ЗахарченкоА. Е.ZakharchenkoA. E.

Москва

Moscow

РеджеповД. Т.RejepovD. T.

Москва

Moscow

https://orcid.org/0000-0003-3396-6816ЕрмолаеваС. А.ErmolaevaS. A.

Ермолаева Светлана Александровна – д-р биол. наук, зав. лабораторией экологии возбудителей инфекций

123098, г. Москва, ул. Гамалеи, 18

Svetlana A. Ermolaeva, Dr. Sci. (Biol.), Head of Laboratory of Ecology of Infectious Agents

18, Gamaleya str., Moscow, 123098

drermolaeva@mail.ruНациональный исследовательский центр эпидемиологии и микробиологии им. Н.Ф. ГамалеиРоссияGamaleya National Research Centre for Epidemiology and MicrobiologyRussian FederationРоссийский университет дружбы народовРоссияPeople’s Friendship UniversityRussian Federation202313042023013843Copyright © Домнин П.А., Захарченко А.Е., Реджепов Д.Т., Ермолаева С.А., 20232023Домнин П.А., Захарченко А.Е., Реджепов Д.Т., Ермолаева С.А.Domnin P.A., Zakharchenko A.E., Rejepov D.T., Ermolaeva S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/2468

Цель работы – оценка применимости системы, основанной на магнитной левитации, для изучения автоагрегации грамотрицательных и грамположительных патогенных бактерий и выяснения механизмов, контролирующих автоагрегацию.

Материалы и методы

Материалы и методы. В работе были использованы вирулентные штаммы Escherichia coli O157:H7, Pseudomonas aeruginosa, Staphylococcus aureus и Listeria monocytogenes. Количество живых бактерий определяли с использованием дифференциального красителя Live/Dead®. Окраску курлей E. coli проводили красителем конго красный.

Результаты

Результаты. Все 4 протестированных вида бактерий в использованных нами условиях формировали автоагрегаты, которые левитировали в объеме жидкости в течение до 72 часов (время наблюдения). Количество живых бактерий в автоагрегатах через 72 часа составляло от 82 (E. coli) до 99% (L. monocytogenes). С использованием описанной системы было показано, что формирование автоагрегатов E. coli зависит от продукции курлей – поверхностных структур, играющих большую роль также и в формировании биопленок.

Заключение

Заключение. Предложена система магнитной левитации, позволяющая изучать молекулярные механизмы автоагрегации и флокуляции патогенных бактерий.

Objective

Objective. To evaluate the potential of magnetic levitation systems when studying the autoaggregation of gram-negative and gram-positive pathogenic bacteria and elucidating mechanisms controlling autoaggregation.

Materials and methods

Materials and methods. Escherichia coli O157:H7, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes were used. The number of alive bacteria was determined using a Live/Dead® dye. E. coli curli were stained with Congo red.

Results

Results. All four tested bacterial species formed autoaggregates that levitated within the liquid volume for up to 72 hours (observation time). After 72 hours, the number of alive bacteria in the autoaggregates ranged from 82% (E. coli) to 99% (L. monocytogenes). The formation of E. coli autoaggregates was shown to depend on the production of curli, which represent surface structures playing an important role in biofilm formation.

Conclusion

Conclusion. The proposed system of magnetic levitation can be used to study molecular mechanisms of bacterial autoaggregation and flocculation.

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The authors declare that there are no conflicts of interest present.