<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">pmj</journal-id><journal-title-group><journal-title xml:lang="ru">Тихоокеанский медицинский журнал</journal-title><trans-title-group xml:lang="en"><trans-title>Pacific Medical Journal</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1609-1175</issn><publisher><publisher-name>TGMU</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.34215/1609-1175-2023-1-55-58</article-id><article-id custom-type="elpub" pub-id-type="custom">pmj-2471</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL RESEARCHES</subject></subj-group></article-categories><title-group><article-title>Ускоренное бактериологическое исследование мочи и диализата при диагностике инфекций мочевыводящих путей у детей</article-title><trans-title-group xml:lang="en"><trans-title>Accelerated bacteriological examination of urine and dialysate in the diagnosis of urinary tract infections in children</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0152-962X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Боронина</surname><given-names>Л. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Boronina</surname><given-names>L. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Боронина Любовь Григорьевна – д-р мед. наук, профессор кафедры клинической лабораторной диагностики и бактериологии</p><p>620149, г. Екатеринбург, ул. Серафимы Дерябиной, 32</p></bio><bio xml:lang="en"><p>Lyubov G. Boronina, Dr. Sci. (Med.), Prof., Dept. of Clinical Laboratory Diagnostics and Bacteriology</p><p>32 Seraphimy Deryabinoy str., Ekaterinburg, 620149</p></bio><email xlink:type="simple">boroninalg@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Саматова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Samatova</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Екатеринбург</p></bio><bio xml:lang="en"><p>Ekaterinburg</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Устюгова</surname><given-names>С. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Ustyugova</surname><given-names>S. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Екатеринбург</p></bio><bio xml:lang="en"><p>Ekaterinburg</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Панова</surname><given-names>С. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Panova</surname><given-names>S. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Екатеринбург</p></bio><bio xml:lang="en"><p>Ekaterinburg</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Куцевалова</surname><given-names>О. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Kutsevalova</surname><given-names>O. Y.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ростов-на-Дону</p></bio><bio xml:lang="en"><p>Rostov-on-Don</p></bio><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Уральский государственный медицинский университет; Областная детская клиническая больница</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Ural State Medical University; Regional Children’s Clinical Hospital</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Областная детская клиническая больница</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Regional Children’s Clinical Hospital</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Национальный медицинский исследовательский центр онкологии</institution><country>Россия</country></aff><aff xml:lang="en"><institution>National Medical Research Centre for Oncology</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>13</day><month>04</month><year>2023</year></pub-date><volume>0</volume><issue>1</issue><fpage>55</fpage><lpage>58</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Боронина Л.Г., Саматова Е.В., Устюгова С.С., Панова С.А., Куцевалова О.Ю., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Боронина Л.Г., Саматова Е.В., Устюгова С.С., Панова С.А., Куцевалова О.Ю.</copyright-holder><copyright-holder xml:lang="en">Boronina L.G., Samatova E.V., Ustyugova S.S., Panova S.A., Kutsevalova O.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.tmj-vgmu.ru/jour/article/view/2471">https://www.tmj-vgmu.ru/jour/article/view/2471</self-uri><abstract><p>Цель: оценить возможность применения ускоренного метода определения этиологии инфекций в моче, диализате и остаточной антимикробной активности на основе технологии лазерного светорассеивания.Материалы и методы. Культурально с января по сентябрь 2019 г. обследовано 106 образцов мочи и 42 образца диализата от детей в возрасте от года до 16 лет с различной патологией мочевыделительной системы, а также находящихся на перитонеальном диализе, на анализаторе ALIFAX HB&amp;L LIGHT (Alifax, Италия), использующем технологию лазерного светорассеивания.Результаты исследования. Через три часа после посева 81 проба показала отрицательный результат (76,4%), 25 образцов – положительный (23,6%). В 38 (90,5%) пробах диализата микроорганизмы не обнаружены, в двух образцах выделены Staphylococcus aureus (104 КОЕ/мл), в одном диализате обнаружены Corynebacterium sp., а также ассоциации Escherichia coli + Candida albicans + Staphylococcus haemolyticus, связанные с колонизацией перитонеального катетера. Бактериурия вызвана представителями порядка Enterobacterales в 39,3% (E. coli, Morganella morganii, Citrobacter amalonaticus, Enterobacter cloacae), неферментирующими грамотрицательными бактериями – в 14,3% (Pseudomonas aeruginosa, Ralstonia picketii, Oligella sp., Acinetobacter baumannii), Enterococcus sp. – 21,4%, S. haemolyticus, Staphylococcus epidermidis – 10,7%, Candida albicans – 3,6%, контаминация: Streptococcus viridians, Corynebacterium sp. – 7,1%. Остаточная антимикробная активность в моче выявлена у 30,1% пациентов.Заключение. Технология лазерного светорассеивания позволяет ускоренно выявлять минимальную концентрацию микроорганизмов в меньшем количестве мочи или диализата, что очень важно для ускоренной диагностики инфекций мочевыводящих путей и осложнений при перитонеальном диализе у детей.</p></abstract><trans-abstract xml:lang="en"><p>Aim. To evaluate the feasibility of an accelerated method for determining the etiology of infections in urine, dialysate and residual antimicrobial activity by using laser light scattering technology.Materials and methods. From January to September 2019, 106 urine samples and 42 dialysate samples from children aged 1–16 with various urinary pathologies and those on peritoneal dialysis underwent culture-based examination on an ALIFAX HB&amp;L LIGHT analyzer (Alifax, Italy) using laser light scattering technology.Results. Three hours after inoculation, 81 samples (76.4%) appeared to be negative and 25 samples (23.6%) proved to be positive. No microorganisms were detected in 38 dialysate samples (90.5%), two samples had Staphylococcus aureus (104 CFU/ml), and one dialysate sample had Corynebacterium sp. and associations of Escherichia coli + Candida albicans + Staphylococcus haemolyticus, associated with peritoneal catheter colonization. Bacteriuria was caused by Enterobacterales in 39.3% (E. coli, Morganella morganii, Citrobacter amalonaticus, Enterobacter cloacae), non-fermenting gram-negative bacteria – in 14.3% (Pseudomonas aeruginosa, Ralstonia picketii, Oligella sp., Acinetobacter baumannii), Enterococcus sp. – 21.4%, S. haemolyticus, Staphylococcus epidermidis – 10.7%, Candida albicans – 3.6%, contamination: Streptococcus viridians, Corynebacterium sp. – 7.1%. Residual antimicrobial activity in urine was detected in 30.1% of patients.Conclusion. The laser light scattering technology enables a minimal concentration of microorganisms to be detected in a smaller amount of urine or dialysate, which is very important for accelerated diagnosis of urinary tract infections and complications of peritoneal dialysis in children.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>бактериурия</kwd><kwd>диализат</kwd><kwd>инфекции мочевыводящих путей</kwd><kwd>диализ</kwd><kwd>дети</kwd></kwd-group><kwd-group xml:lang="en"><kwd>bacteriuria</kwd><kwd>dialysate</kwd><kwd>urinary tract infections</kwd><kwd>dialysis</kwd><kwd>children</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">авторы заявляют о финансировании проведенного исследования из собственных средств.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Коровина Н.А., Захарова И.Н., Мумладзе Э.Б., Горяйнова А.Н. Инфекция мочевой системы у детей: современные подходы к диагностике и лечению. Русский медицинский журнал. 2007;(2):1533–42.</mixed-citation><mixed-citation xml:lang="en">Korovina NA, Zaharova IN, Mumladze EB, Goryajnova AN. Urinary tract infection in children: modern approaches to diagnosis and treatment. Russkij Medicinskij Zhurnal. 2007;(2):1533–42 (In Russ.).</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Бактериологический анализ мочи. Методические рекомендации. М.: Федерация лабораторной медицины, 2013.</mixed-citation><mixed-citation xml:lang="en">Bacteriological analysis of urine. Guidelines. Moscow: Federation of Laboratory Medicine; 2013 (In Russ.).</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Рекомендации по ведению больных с инфекциями почек, мочевых путей и мужских половых органов (European Association of Urology, 2008). URL: http://www.antibiotic.ru/index.phppage=17 (дата обращения: 05.05.2020).</mixed-citation><mixed-citation xml:lang="en">Recommendations for the management of patients with kidney, urinary tract and male genital infections. (European Association of Urology, 2008) (In Russ.). URL: http://www.antibiotic.ru/index.phppage=17 (Accessed 05.05.2020).</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Гилязова Д.Г. Ускорение бактериологического анализа с помощью технологии Alifax: результат за часы. Справочник заведующего КДЛ. 2014;(3):69–73.</mixed-citation><mixed-citation xml:lang="en">Gilyazova DG. Acceleration of bacteriological analysis with Alifax technology: result in hours. Spravochnik Zaveduyushchego KLD. 2014;(3):69–73 (In Russ.).</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Ilki A, Bekdemir P, Ulger N, Soyletir G. Rapid reporting of urine culture results: impact of the uro-quick screening system. New microbiologica. 2010;33(2):147–53.</mixed-citation><mixed-citation xml:lang="en">Ilki A, Bekdemir P, Ulger N, Soyletir G. Rapid reporting of urine culture results: impact of the uro-quick screening system. New microbiologica. 2010;33(2):147–53.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Ballabio C, Venturi N, Sala MR, Mocarelli P, Brambilla P. Evaluation of an automated method for urine culture screening. Microbiologia Medica. 2010;25(3):178–80.</mixed-citation><mixed-citation xml:lang="en">Ballabio C, Venturi N, Sala MR, Mocarelli P, Brambilla P. Evaluation of an automated method for urine culture screening. Microbiologia Medica. 2010;25(3):178–80.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Isenberg HD. Essential procedures for clinical microbiology. Washington, D.C.: ASM PRESS; 1998.</mixed-citation><mixed-citation xml:lang="en">Isenberg HD. Essential procedures for clinical microbiology. Washington, D.C.: ASM PRESS; 1998.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
