pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMU10.34215/1609-1175-2024-4-69-75pmj-2832Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESМетаболически ориентированное действие фукоидана из бурой водоросли Sargassum feldmannii на формирование колоний клеток рака молочной железы человека MDA-MB-231Metabolically-oriented effect of fucoidan from Sargassum feldmannii brown algae on the mda-mb-231 human breast cancer cellsМаляренкоО. С.MalyarenkoO. S.

Владивосток

Vladivostok

ЗуеваА. О.ZuevaA. O.

Владивосток

Vladivostok

УсольцеваР. В.UsoltsevaR. V.

Владивосток

Vladivostok

СильченкоА. С.SilchenkoA. S.

Владивосток

Vladivostok

https://orcid.org/0000-0002-5905-2046ЕрмаковаС. П.ErmakovaS. P.

Светлана Павловна Ермакова, д-р хим. наук, доцент, зав. лабораторией

лаборатория химии ферментов

690022; просп. 100 лет Владивостоку, 159; Владивосток

тел.: (423) 231-07-05

Svetlana P. Ermakova, Dr. Sci. (Med.), Head of the Laboratory

Laboratory of Enzyme Chemistry

690022; 159, Prospect 100-lyet Vladivostoku; Vladivostok

phone: (423) 231-07-05

swetlana_e@mail.ruТихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАНРоссияG.B. Elyakov Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of Russian Academy of SciencesRussian Federation202416022025046975Copyright © Маляренко О.С., Зуева А.О., Усольцева Р.В., Сильченко А.С., Ермакова С.П., 20252025Маляренко О.С., Зуева А.О., Усольцева Р.В., Сильченко А.С., Ермакова С.П.Malyarenko O.S., Zueva A.O., Usoltseva R.V., Silchenko A.S., Ermakova S.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/2832   Цель

   Цель: изучить влияние фукоидана из бурой водоросли S. feldmannii (SfF2) на метаболизм клеток рака молочной железы человека MDA-MB-231.

   Материалы и методы

   Материалы и методы: определен биоэнергетический потенциал клеток MDA-MB-231 под воздействием различных факторов (инсулина, эпидермального фактора роста, форболового эфира и рентгеновского излучения), стимулирующих прогрессирование опухолей. С использованием метода мягких агаров определено метаболически ориентированное действие фукоидана SfF2 (200 мкг/мл) на процесс формирования колоний клеток рака молочной железы человека MDA-MB-231 при нормальных условиях и под воздействием различных канцерогенных факторов.

   Результаты

   Результаты: установлено, что SfF2 снижал количество колоний MDA-MB-231 клеток, индуцированных инсулином, эпидермальным фактором роста (EGF) и 12-O-тетрадеканоил-форбол-13-ацетатом (TPA), более чем на 50 % по сравнению с контрольными клетками.

   Заключение

   Заключение: использование фукоидана в качестве дополнения к базовой терапии представляется перспективной стратегией для повышения эффективности противоопухолевой терапии.

   Objective

   Objective. To study the effect of fucoidan from brown algae S. feldmannii (SfF2) on the metabolism of MDA-MB-231 breast cancer cells.

   Materials and methods

   Materials and methods. The bioenergetic potential of MDA-MB-231 cells was assessed under the influence of various factors (insulin, epidermal growth factor, phorbol ester, and X-rays) stimulating tumor progression. The metabolically oriented effect of fucoidan SfF2 (200 µg/ml) on the colony formation process of MDA-MB-231 human breast cancer cells was determined using the soft agar method under normal conditions and under the influence of various carcinogenic factors.

   Results

   Results. Fucoidan from brown algae S. feldmannii was found to reduce the number of colonies of MDA-MB-231 cells induced by insulin, epidermal growth factor (EGF), and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) by more than 50 % compared to control cells.

   Conclusion

   Conclusion. The use of fucoidan as an adjunct to standard therapy appears to be a promising strategy for enhancing the efficacy of anticancer treatment.

сульфатированные полисахаридыбурые водорослиметаболизмопухолевые клеткиsulfated polysaccharidesbrown algaemetabolismtumor cellsРабота выполнена при финансовой поддержке гранта РНФ № 21-14-00321The work was carried out with the financial support of the Russian Science Foundation grant No. 21-14-00321ReferencesWarburg O. On the origin of cancer cells. Science. 1956;123 (3191):309–314. doi: 10.1126/science.123.3191.309Warburg O. On the origin of cancer cells. Science. 1956;123 (3191):309–314. doi: 10.1126/science.123.3191.309Dhillon AS, Hagan S, Rath O, Kolch W. MAP kinase signalling pathways in cancer. Oncogene. 2007;26(22):3279–3290. doi: 10.1038/sj.onc.1210421Dhillon AS, Hagan S, Rath O, Kolch W. MAP kinase signalling pathways in cancer. Oncogene. 2007;26(22):3279–3290. doi: 10.1038/sj.onc.1210421Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646–674. doi: 10.1016/j.cell.2011.02.013Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646–674. doi: 10.1016/j.cell.2011.02.013Kishton RJ, Rathmell JC. Novel therapeutic targets of tumor metabolism. Cancer J. 2015;21(2):62–69. doi: 10.1097/PPO.0000000000000099Kishton RJ, Rathmell JC. Novel therapeutic targets of tumor metabolism. Cancer J. 2015;21(2):62–69. doi: 10.1097/PPO.0000000000000099Usoltseva RV, Shevchenko NM, Malyarenko OS, Ishina IA, Ivannikova SI, Ermakova SP. Structure and anticancer activity of native and modified polysaccharides from brown alga Dictyota dichotoma. Carbohydr. Polym. 2018;180:21–28. doi: 10.1016/j.carbpol.2017.10.006Usoltseva RV, Shevchenko NM, Malyarenko OS, Ishina IA, Ivannikova SI, Ermakova SP. Structure and anticancer activity of native and modified polysaccharides from brown alga Dictyota dichotoma. Carbohydr. Polym. 2018;180:21–28. doi: 10.1016/j.carbpol.2017.10.006Lee NY, Ermakova SP, Choi HK, Kusaykin MI, Shevchenko NM, Zvyagintseva TN, Choi HS. Fucoidan from Laminaria cichorioides inhibits AP-1 transactivation and cell transformation in the mouse epidermal JB6 cells. Mol. Carcinog. 2008;47(8):629–637. doi: 10.1002/mc.20428Lee NY, Ermakova SP, Choi HK, Kusaykin MI, Shevchenko NM, Zvyagintseva TN, Choi HS. Fucoidan from Laminaria cichorioides inhibits AP-1 transactivation and cell transformation in the mouse epidermal JB6 cells. Mol. Carcinog. 2008;47(8):629–637. doi: 10.1002/mc.20428Vishchuk OS, Sun H, Wang Z, Ermakova SP, Xiao J, Lu T, Xue P, Zvyagintseva TN, Xiong H, Shao C, Yan W, Duan Q, Zhu F. PDZ-binding kinase/T-LAK cell-originated protein kinase is a target of the fucoidan from brown alga Fucus evanescens in the prevention of EGF-induced neoplastic cell transformation and colon cancer growth. Oncotarget. 2016;7(14):18763–18773. doi: 10.18632/oncotarget.7708Vishchuk OS, Sun H, Wang Z, Ermakova SP, Xiao J, Lu T, Xue P, Zvyagintseva TN, Xiong H, Shao C, Yan W, Duan Q, Zhu F. PDZ-binding kinase/T-LAK cell-originated protein kinase is a target of the fucoidan from brown alga Fucus evanescens in the prevention of EGF-induced neoplastic cell transformation and colon cancer growth. Oncotarget. 2016;7(14):18763–18773. doi: 10.18632/oncotarget.7708Luthuli S, Wu S, Cheng Y, Zheng X, Wu M, Tong H. Therapeutic Effects of Fucoidan : A Review on Recent Studies. Mar. Drugs. 2019;17(9). doi: 10.3390/md17090487Luthuli S, Wu S, Cheng Y, Zheng X, Wu M, Tong H. Therapeutic Effects of Fucoidan : A Review on Recent Studies. Mar. Drugs. 2019;17(9). doi: 10.3390/md17090487Usoltseva RV, Anastyuk SD, Surits VV, Shevchenko NM, Thinh PD, Zadorozhny PA, Ermakova SP. Comparison of structure and in vitro anticancer activity of native and modified fucoidans from Sargassum feldmannii and S. duplicatum. IJBM. 2019;124:220–228. doi: 10.1016/j.ijbiomac.2018.11.223Usoltseva RV, Anastyuk SD, Surits VV, Shevchenko NM, Thinh PD, Zadorozhny PA, Ermakova SP. Comparison of structure and in vitro anticancer activity of native and modified fucoidans from Sargassum feldmannii and S. duplicatum. IJBM. 2019;124:220–228. doi: 10.1016/j.ijbiomac.2018.11.223Davis RT, Blake K, Ma D, Gabra MBI, Hernandez GA, Phung AT, Yang Y, Maurer D, Lefebvre A, Alshetaiwi H, Xiao Z, Liu J, Locasale JW, Digman MA, Mjolsness E, Kong M, Werb Z, Lawson DA. Transcriptional diversity and bioenergetic shift in human breast cancer metastasis revealed by single-cell RNA sequencing. Nat Cell Biol. 2020;22(3):310–320. doi: 10.1038/s41556-020-0477-0Davis RT, Blake K, Ma D, Gabra MBI, Hernandez GA, Phung AT, Yang Y, Maurer D, Lefebvre A, Alshetaiwi H, Xiao Z, Liu J, Locasale JW, Digman MA, Mjolsness E, Kong M, Werb Z, Lawson DA. Transcriptional diversity and bioenergetic shift in human breast cancer metastasis revealed by single-cell RNA sequencing. Nat Cell Biol. 2020;22(3):310–320. doi: 10.1038/s41556-020-0477-0Chen LM, Yang PP, Al Haq AT, Hwang PA, Lai YC, Weng YS, Chen MA, Hsu HL. Oligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in mice. J Biomed Sci. 2022;29(1):70. doi: 10.1186/s12929-022-00855-6Chen LM, Yang PP, Al Haq AT, Hwang PA, Lai YC, Weng YS, Chen MA, Hsu HL. Oligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in mice. J Biomed Sci. 2022;29(1):70. doi: 10.1186/s12929-022-00855-6Zhao M, Jung Y, Jiang Z, Svensson KJ. Regulation of energy metabolism by receptor tyrosine kinase ligands. Front Physiol. 2020;11:354. doi: 10.3389/fphys.2020.00354Zhao M, Jung Y, Jiang Z, Svensson KJ. Regulation of energy metabolism by receptor tyrosine kinase ligands. Front Physiol. 2020;11:354. doi: 10.3389/fphys.2020.00354Malyarenko OS, Ermakova SP. Fucoidans: anticancer activity and molecular mechanisms of action. J. Venkatesan, S. Anil, S.K. Kim (Eds.). Seaweed polysaccharides, Elsevier, Amsterdam, Netherlands 2017;175–203. doi: 10.1016/B978-0-12-809816-5.00010-4Malyarenko OS, Ermakova SP. Fucoidans: anticancer activity and molecular mechanisms of action. J. Venkatesan, S. Anil, S.K. Kim (Eds.). Seaweed polysaccharides, Elsevier, Amsterdam, Netherlands 2017;175–203. doi: 10.1016/B978-0-12-809816-5.00010-4Sim SY, Shin YE, Kim HK. Fucoidan from Undaria pinnatifida has anti-diabetic effects by stimulation of glucose uptake and reduction of basal lipolysis in 3T3-L1 adipocytes. Nutr Res. 2019;65:54–62. doi: 10.1016/j.nutres.2019.02.002Sim SY, Shin YE, Kim HK. Fucoidan from Undaria pinnatifida has anti-diabetic effects by stimulation of glucose uptake and reduction of basal lipolysis in 3T3-L1 adipocytes. Nutr Res. 2019;65:54–62. doi: 10.1016/j.nutres.2019.02.002Shan X, Wang X, Jiang H, Cai C, Hao J, Yu G. Fucoidan from Ascophyllum nodosum Suppresses Postprandial Hyperglycemia by Inhibiting Na(+)/Glucose Cotransporter 1 Activity. Mar. Drugs. 2020;18(9). doi: 10.3390/md18090485Shan X, Wang X, Jiang H, Cai C, Hao J, Yu G. Fucoidan from Ascophyllum nodosum Suppresses Postprandial Hyperglycemia by Inhibiting Na(+)/Glucose Cotransporter 1 Activity. Mar. Drugs. 2020;18(9). doi: 10.3390/md18090485

The authors declare that there are no conflicts of interest present.