pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMUpmj-582Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESМатриксные металлопротеинзы 14 и 17 как факторы ревасуляризации при аденомиозеMatrix metalloproteinases 14 and 17 as factors of revascularization in adenomyosisТкачеваЕ. В.TkachyovaE. V.tkacheva1974@gmail.comГореликМ. З.GorelikM. Z.noemail@neicon.ruДюйзенИ. В.DyuizenI. V.noemail@neicon.ruТихоокеанский государственный медицинский университетРоссияPacific State Medical UniversityRussian FederationПриморское краевое патологоанатомическое бюроРоссияrimorsky Regional Patho-anatomical DepartmentRussian Federation201528062015026972Copyright © Ткачева Е.В., Горелик М.З., Дюйзен И.В., 20152015Ткачева Е.В., Горелик М.З., Дюйзен И.В.Tkachyova E.V., Gorelik M.Z., Dyuizen I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/582

С помощью иммуногистохимического метода изучали локализацию матриксных металлопротеиназ (ММР) в эуто-пическом эндометрии женщин с аденомиозом. Обнаружены селективные изменения тканевой локализации и активности ММР14 и ММР17 по сравнению с контрольными образцами эндометрия здоровых женщин в сходной стадии овариальноменструального цикла. В соединительной ткани эндометрия при аденомиозе нарастала активность ММР14, в стенке кровеносных сосудов экспрессировались ММР14 и ММР17, а в клетках маточных желез появллась несвойственная данной стадии цикла ММР17. В совокупности данные изменения могут нарушать опорные и сигнальные свойства ткани, создавая благоприятные предпосылки для эндометриоидной гетеротопии. Ключевые слова: внутренний эндометриоз, матка, протеолитические ферменты, шкала иммунореактивности.

Background. The localization of matrix metalloproteinases (MMPs) 14 and 17 was studied in eutopic en-dometrial tissue of women with internal endometriosis. Methods. The study included 20 women of childbearing age suffering from dysfunctional uterine bleeding and infertility. The control group consisted of patients with no signs of endometriosis, who had a history of pregnancy. The endometrial biopsy was detected the presence and location of MMP14 and MMR17 by immunohis-tochemistry. Results. All women with endometriosis diagnosed glandular endometrial hyperplasia and tissue changes observed selective localization and activity of MMP14 and MMR17. In the endometrial stroma adenomy-osis was a growing activity of MMP14 in the wall of blood vessels and MMP14 expressed MMR17, and in the cells of uterine glands appearing not peculiar stage of the cycle MMR17. Conclusions. Eutopic endometrial tissue of women with adenomyosis are characterized by changing the profile of activity of some MMPs. Together, these changes may disrupt the support and signaling proper-ties of the fabric, creating favorable conditions for endometrioid heterotopia.

внутренний эндометриозматкапротеолитические ферментышкала иммунореактивностиinternal endometriosisuterusproteolytic enzymesimmunoreactive scaleReferencesКоган Е.А., Унанян А.Л., Демура Т.А. [и др.] Клинико-морфологические параллели и молекулярные аспекты aденомиоза // Архив патологии. 2008. № 5. С. 8-12.Коган Е.А., Унанян А.Л., Демура Т.А. [и др.] Клинико-морфологические параллели и молекулярные аспекты aденомиоза // Архив патологии. 2008. № 5. С. 8-12.Collette T., Bellehumeur C., Kats R. [et al.] Evidence for an increased release of proteolytic activity by the eutopic endometrial tissue in women with endometriosisand for involvement of matrix metalloproteinase-9 // Human Reproduction. 2004. Vol. 19, No. 6. Р. 1257-1264.Collette T., Bellehumeur C., Kats R. [et al.] Evidence for an increased release of proteolytic activity by the eutopic endometrial tissue in women with endometriosisand for involvement of matrix metalloproteinase-9 // Human Reproduction. 2004. Vol. 19, No. 6. Р. 1257-1264.Coyle R.C., Latimer A., Jessen J.R. Membrane-type 1 matrix metalloproteinase regulates cell migration during zebrafish gastrulation: Evidence for an interaction with non-canonical Wnt signaling // Experimental cell research. 2008. No. 314. Р. 2150-2162.Coyle R.C., Latimer A., Jessen J.R. Membrane-type 1 matrix metalloproteinase regulates cell migration during zebrafish gastrulation: Evidence for an interaction with non-canonical Wnt signaling // Experimental cell research. 2008. No. 314. Р. 2150-2162.Curry J.T.E., Osteen K.G. Cyclic changes in the matrix metalloproteinase system in the ovary and uterus // Biology of Reproduction. 2001. No. 64. Р 1285-1296.Curry J.T.E., Osteen K.G. Cyclic changes in the matrix metalloproteinase system in the ovary and uterus // Biology of Reproduction. 2001. No. 64. Р 1285-1296.Egeblad M., Werb Z. New functions for the matrix metallopro-teinases in cancer progression // Nat Rev Cancer. 2002. No. 2. Р 161-174.Egeblad M., Werb Z. New functions for the matrix metallopro-teinases in cancer progression // Nat Rev Cancer. 2002. No. 2. Р 161-174.English William R., Xose S., Jose M.P [et al.] Membrane type 4 matrix metalloproteinase (MMP17) has tumor necrosis factor-a convertase activity but does not activate pro-MMP2 // The Journal of Biological Chemistry. 2000. Vol. 275, No. 19. Р 14046-14055.English William R., Xose S., Jose M.P [et al.] Membrane type 4 matrix metalloproteinase (MMP17) has tumor necrosis factor-a convertase activity but does not activate pro-MMP2 // The Journal of Biological Chemistry. 2000. Vol. 275, No. 19. Р 14046-14055.Freitas S., Meduri G., Le Nestour E. Expression of metalloproteinases and their inhibitors in blood vessels in human endometrium // Biology of Reproduction. 1999. No. 61. Р. 1070-1082.Freitas S., Meduri G., Le Nestour E. Expression of metalloproteinases and their inhibitors in blood vessels in human endometrium // Biology of Reproduction. 1999. No. 61. Р. 1070-1082.Gilabert-Estelle J., Estelle A., Gilabert J. Expression of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis // Human Reproduction. 2003. Vol. 18, No. 7. Р 1516-1522.Gilabert-Estelle J., Estelle A., Gilabert J. Expression of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis // Human Reproduction. 2003. Vol. 18, No. 7. Р 1516-1522.Giudice L.C, Tazuke S.I., Swiersz L. Status of current research on endometriosis // Journal Reprod Me. 1998. No. 43. Р 252-262.Giudice L.C, Tazuke S.I., Swiersz L. Status of current research on endometriosis // Journal Reprod Me. 1998. No. 43. Р 252-262.Holmbeck K., Bianco P., Yamada S. [et al.] MT1-MMP: a tethered collagenase // Journal of Cellular Physiology. 2004. No. 200. Р. 11-19.Holmbeck K., Bianco P., Yamada S. [et al.] MT1-MMP: a tethered collagenase // Journal of Cellular Physiology. 2004. No. 200. Р. 11-19.Laudanski P., Szamatowicz J., Ramel P. Matrix metalloproteinase-13 and membrane type-1 matrix metalloproteinase in peritoneal fluid of women with endometriosis // Gynecological Endocrinology. 2005. Vol. 21, No. 2. Р. 106-110.Laudanski P., Szamatowicz J., Ramel P. Matrix metalloproteinase-13 and membrane type-1 matrix metalloproteinase in peritoneal fluid of women with endometriosis // Gynecological Endocrinology. 2005. Vol. 21, No. 2. Р. 106-110.Machado D.E., Berardo P.T., Palmero C.Y. Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases // Journal of Experimental & Clinical Cancer Research. 2010. Vol. 29, No. 4. Р. 1-9.Machado D.E., Berardo P.T., Palmero C.Y. Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases // Journal of Experimental & Clinical Cancer Research. 2010. Vol. 29, No. 4. Р. 1-9.Marion W., Kramer J., Schem C. [et al.] Differential expression of MMP-2, MMP-9 and PCNA in endometriosis and endometrial carcinoma // European Journal of Obstetrics & Gynecology and Reproductive Biology. 2012. No. 160. Р. 74-78.Marion W., Kramer J., Schem C. [et al.] Differential expression of MMP-2, MMP-9 and PCNA in endometriosis and endometrial carcinoma // European Journal of Obstetrics & Gynecology and Reproductive Biology. 2012. No. 160. Р. 74-78.Roopali R., Yang J., Moses M.A. Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer// Journal Oncology. 2009. Vol. 27, No. 31. Р. 5287-5297.Roopali R., Yang J., Moses M.A. Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer// Journal Oncology. 2009. Vol. 27, No. 31. Р. 5287-5297.Sounni N.E., Devy L., Hajitou A. [et al.] MT1- MMP expression promotes tumor growth and angiogenesis through an up-regulation of vascular endothelial growth factor expression // FASEB Journal. 2002. No. 16. Р. 555-564.Sounni N.E., Devy L., Hajitou A. [et al.] MT1- MMP expression promotes tumor growth and angiogenesis through an up-regulation of vascular endothelial growth factor expression // FASEB Journal. 2002. No. 16. Р. 555-564.

The authors declare that there are no conflicts of interest present.