pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMUpmj-681Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESВЛИЯНИЕ ПЕРИФЕРИЧЕСКИ-ИНДУЦИРОВАННОГО НЕЙРОВОСПАЛЕНИЯ НА КОГНИТИВНЫЕ ФУНКЦИИ У МОЛОДЫХ И СТАРЫХ МЫШЕЙEFFECT OF PERIPHERICALLY-INDUCED NEUROINFLAMMATION ON COGNITIVE FUNCTION IN YOUNG AND OLD MICEТыртышнаяА. А.TyrtyshnayaA. A.dr.anna.kelvin@gmail.comЗозуляА. А.ZozulyaA. A.noemail@neicon.ruИнститут биологии моря им. А.В. Жирмунского ДВО РАН; Дальневосточный федеральный университетРоссияInstitute of Marine Biology named after A.V. Zhirmunskiy FEB RAS; Far Eastern Federal UniversityRussian FederationИнститут биологии моря им. А.В. Жирмунского ДВО РАНРоссияInstitute of Marine Biology named after A.V. Zhirmunskiy FEB RASRussian Federation201428062014022326Copyright © Тыртышная А.А., Зозуля А.А., 20142014Тыртышная А.А., Зозуля А.А.Tyrtyshnaya A.A., Zozulya A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/681

Оценивали влияние периферически индуцированного нейровоспаления на выраженности когнитивных нарушений в зависимости от возраста у мышей. Нейровоспаление индуцировали путем внутрибрюшинного введения 3- и 18-месячным животным бактериальных липополисахаридов Escherichia coli 0111:B4 (Sigma Aldrich) в дозировке 5 мг/кг. У 18-месячных мышей выявлены более выраженные когнитивный дефицит и снижение локомоторной активности по сравнению с группой «молодых» животных. Иммуногистохимическими методами в гиппокампе у 18-месячных мышей показаны более выраженные активация микроглии и астроглиоз.

The article is devoted to experimental evaluation of the impact of peripherically-induced neuroinflammation on the severity of cognitive impairment, depending on age. Methods. Neuroinflammation has been induced in the 3- and 18-month-old white male mice with an intraperitoneal injection of bacterial lipopolysaccharides Escherichia coli 0111: B4 (Sigma Aldrich) 5 mg/kg. After 24 hours, the researchers determined locomotor activity and a working memory in Y-maze. Then the test animals were taken out of the experience and assessed the expression of the proteins GFAP and OX-42 on transverse hippocampal sections. Results. In 18-month-old mice there have been revealed more severe cognitive deficit and decreased locomotor activity compared with the group of “young” test animals. Immunohistochemical methods in hippocampus of 18-month-old mice showed more pronounced activation of microglia and astrogliosis. Conclusions. Aging is accompanied by increased tendency to neuroinflammation. Stimulation of the immune system by bacterial endotoxin increases the severity of inflammatory reactions in the brain of aged mice compared to young animals, regularly provoking neurodegeneration and cognitive deficit.

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The authors declare that there are no conflicts of interest present.