pmjТихоокеанский медицинский журналPacific Medical Journal1609-1175TGMUpmj-748Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESРОЛЬ 15-ГИДРОКСИЭЙКОЗАТЕТРАЕНОВОЙ КИСЛОТЫ В ИНАКТИВАЦИИ КАЛИЕВЫХ КАНАЛОВ И СПАЗМЕ ВНУТРЕННЕЙ СОННОЙ АРТЕРИИ ПРИ ГИПОКСИИHYPOXIA-INDUCED 15-HETE ENHANCES THE CONSTRICTION OF INTERNAL CAROTID ARTERIES BY DOWN-REGULATING POTASSIUM CHANNELSЯнмейЖу.YanmeiZ.noemail@neicon.ruЛиЧ.LiC.noemail@neicon.ruВеньянЛ.WenjuanL.noemail@neicon.ruВейжиВ.WeizhiW.noemail@neicon.ruДалингЖу.DalingZ.noemail@neicon.ruЮланЖу.YulanZ.ylz1962@yahoo.com.cnВторой аффилированный госпитальРоссияSecond Affiliated Hospital of Harbin Medical UniversityRussian FederationФармацевтический колледж Харбинского медицинского университетаРоссияCollege of Pharmacy of Harbin Medical UniversityRussian Federation201328122013046367Copyright © Янмей Ж., Ли Ч., Веньян Л., Вейжи В., Далинг Ж., Юлан Ж., 20132013Янмей Ж., Ли Ч., Веньян Л., Вейжи В., Далинг Ж., Юлан Ж.Yanmei Z., Li C., Wenjuan L., Weizhi W., Daling Z., Yulan Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tmj-vgmu.ru/jour/article/view/748

Тяжелая гипоксия вызывает спазм внутренней сонной артерии, который опасен ишемическим повреждением головного мозга. Некоторые метаболиты могут спровоцировать вазоконстрикцию, однако механизм их действия до конца не расшифрован. В эксперименте на крысах исследовали влияние 15-гидроксиэйкозатетраеновой кислоты (15-HETE), продуцируемой 15-липоксигеназой, на тонус сосудов при гипоксии. Обнаружено повышение уровня 15-липоксигеназы в эндотелии и гладкомышечных клетках внутренней сонной артерии. 15-HETE обусловливала дозозависимый спазм сосуда, а также снижение активности потенциалзависимых калиевых каналов. Таким образом, инактивация калиевых каналов 15-HETE при гипоксии приводит к ослаблению реполяризации активных потенциалов и притоку калия в клетки, что вызывает повышение тонуса гладкой мускулатуры сосудов и спазм внутренней сонной артерии.

Severe hypoxia induces the constriction of internal carotid arteries (ICA), which worsens ischemic stroke in the brain. A few metabolites are presumably involved in hypoxic vasoconstriction, however, less is known about how such molecules provoke this vasoconstriction. We have investigated the influence of 15-hydroxyeicosatetrienoic acid (15-HETE) produced by 15-lipoxygenase (15-LOX) on vasoconstriction during hypoxia. As showed in our results, 15-LOX level increases in ICA endothelia and smooth muscles. 15-HETE enhances the tension of ICA ring in a dose-dependent manner, as well as attenuates the activity and expression of voltage-gated potassium (Kv) channels. Therefore, the down-regulation of Kv channels by 15-HETE during hypoxia weakens the repolarization of action potentials and causes a dominant influx of calcium to enhance smooth muscle tension and ICA constriction.

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The authors declare that there are no conflicts of interest present.