Molecular genetic analysis of genome of Glubinnoe/2004 high virulent strain of tick-bone encephalitis
Abstract
The authors present molecular genetic analysis of sequencing
of genome of Glubinnoe/2004 high virulent strain of tickbone
encephalitis (Far Eastern sub-type). Upon comparing this genome
with the genomes of other strains of the Far Eastern, Siberian,
European sub-types, the authors indicate that the Glubinnoy/2004
strain has a number of mutations in the unstructured virus proteins,
virus polyprotein processing sites and promoter sequences of the
genome. These mutations are likely to cause accelerated replication
and maturation of the virus. As shown, the secondary structure of
promotor 5end genome domain and the position of transmembrane
domains in NS2A protein distinguish the FE-subtype strains from
the Siberian and European subtypes of the tick-bone encephalitis.
of genome of Glubinnoe/2004 high virulent strain of tickbone
encephalitis (Far Eastern sub-type). Upon comparing this genome
with the genomes of other strains of the Far Eastern, Siberian,
European sub-types, the authors indicate that the Glubinnoy/2004
strain has a number of mutations in the unstructured virus proteins,
virus polyprotein processing sites and promoter sequences of the
genome. These mutations are likely to cause accelerated replication
and maturation of the virus. As shown, the secondary structure of
promotor 5end genome domain and the position of transmembrane
domains in NS2A protein distinguish the FE-subtype strains from
the Siberian and European subtypes of the tick-bone encephalitis.
Keywords
578.833:57.063.8.575.224.234
About the Authors
Е. Чаусов
НИИ эпидемиологии и микробиологии СО РАМН
Russian Federation
Russian Federation
В. Терновой
ГНЦ вирусологии и биотехнологии "Вектор"
Russian Federation
Russian Federation
Е. Протопопова
ГНЦ вирусологии и биотехнологии "Вектор"
Russian Federation
Russian Federation
Г. Леонова
НИИ эпидемиологии и микробиологии СО РАМН
Russian Federation
Russian Federation
В. Локтев
ГНЦ вирусологии и биотехнологии "Вектор"
Russian Federation
Russian Federation
E. V. Chausov
Research Institute of Epidemiology and Microbiology, SB RAMS
Russian Federation
Russian Federation
V. A. Ternovoy
State Research Centre
of Virology and Biotechnology "Vector"
Russian Federation
Russian Federation
E. V. Protopopova
State Research Centre
of Virology and Biotechnology "Vector"
Russian Federation
Russian Federation
G. N. Leonova
Research Institute of Epidemiology and Microbiology, SB RAMS
Russian Federation
Russian Federation
V. B. Loktev
State Research Centre
of Virology and Biotechnology "Vector"
Russian Federation
Russian Federation
References
1. Леонова Г.Н. Клещевой энцефалит в Приморском крае. Владивосток: Дальнаука, 1997. 190 с.
2. Погодина В.В., Фролова М.П., Ерман Б.А. Хронический клещевой энцефалит. Новосибирск: Наука, 1986. 232 с.
3. Chausov E.V., Ternovoi V.A., Protopopova E.V. et al. Variability of the Tick-Borne Encephalitis Virus Genome in the 5' Noncoding Region Derived from Ticks Ixodes persulcatus and Ixodes pavlovskyi in Western Siberia // Vector Borne Zoonotic Dis. 2009. Oct 30. [Epub ahead of print].
4. Corver J., Lenches E., Smith K. et al. Fine mapping of a cis-acting sequence element in yellow fever virus RNA that is required for RNA replication and cyclization // Journal Virol. 2003. Vol. 77. P. 2265- 2270.
5. Ecker M. Allison S.L., Meixner T., Heinz F.X. Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia // J. Gen. Virol. 1999. Vol. 80. P. 179-185.
6. Filomatori C.V., Lodeiro M.F., Alvarez D.E. et al. 5' RNA enement promotes dengue virus synthesis on a circular genome // Genes Dev. 2006. Vol. 20. P. 2238-2249.
7. Hayasaka D., Gritsun T.S., Yoshii K. et al. Amino acid changes responsible for attenuation of neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus // J. Gen. Virol. 2004. Vol. 85. P. 1007-1018.
8. Holzmann H., Stiasny K., Ecker M. et al. Characterization of monoclonal antibody-escape mutants of tick-borne encephalitis virus with reduced neuroinvasiveness in mice // J. Gen. Virol. 1997. Vol. 78. P. 31-37.
9. Khromykh A.A., Kondratieva N., Sgro J-Y. et al. Significance in replication of the terminal nucleotides of the flavivirus genome // J. Virol. 2003. Vol. 77 (19). P. 10623-10629.
10. Mackensie J.M., Khromykh A.A., Jones M.K., Westaway E.G. Subcelluar localization and some biochemical properties of the Flavivirus Kunjin nonstructural proteins NS2A and NS4A // Virology. 1998. Vol. 245. P. 203-215.
11. Mandl C.W., Alison S.L., Holzman H. et al. Attenuation of tickborne encephalitis by sructure-based site-specific mutagenesis of putative flavivirus receptor binding site // J. Virol.- 2000. Vol. 74 (20). P. 9601-9609.
12. Park G.S., Morris K.L., Hallett R.G. et al. Identification of Residues Critical for the Interferon Antagonist Function of Langat Virus NS5 Reveals a Role for the RNA-Dependent RNA Polymerase Domain // J. Virol. 2007. Vol. 81. P. 6936-6946.
13. Tamura K., Dudley J., Nei M. & Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 // Molecular Biology and Evolution. 2007. Vol. 24. P. 1596-1599.
14. Ternovoi V.A., Protopopova E.V., Chausov E.V. et al. Novel variant of Tick-borne encephalitis virus, Russia // Emerg. Infect. Dis. 2007. Vol. 13. P. 1574-1578.
Review
For citations:
, , , , , Chausov E.V., Ternovoy V.A., Protopopova E.V., Leonova G.N., Loktev V.B. Molecular genetic analysis of genome of Glubinnoe/2004 high virulent strain of tick-bone encephalitis. Pacific Medical Journal. 2010;(3):27-30. (In Russ.)
Views:
186
Email this article 