STUDYING MECHANISMS OF APOPTOSIS -MODULATING ACTIVITY OF THERMORESISTANT TOXIN YERSINIA PSEUDOTUBERCULOSIS AND CORRECTIVE EFFECT OF FAR- EASTERN HOLOTHURIANS -DERIVED EXTRACT IN RAT NEUTROPHILS IN VITRO
Abstract
The thermoresistant lethal toxin Y. pseudotuberculosis
has concentration-dependent effect on the activity of antioxidant
enzymes, apoptosis and viability of neutrophils and binding of surface
cell receptors and concanavalin A in vitro. This effect also depends
on the duration of hatching. The results of experiments on
simultaneous introduction of this toxin with phosphodiesterase inhibitor
(caffeine) and commercial catalase are indicative of the fact
that the effects from the toxin depend on cyclic adenosine monophosphate
content in cells. The apoptosis progresses when the antioxidant
enzyme increase. The extract derived from the Far Eastern
holothurians allows preventing inhibitory activity of this toxin on
binding cell receptors to concanavalin A and inducing apoptosis
and activity of antioxidant enzymes, thus increasing functional activity
of neutrophils.
has concentration-dependent effect on the activity of antioxidant
enzymes, apoptosis and viability of neutrophils and binding of surface
cell receptors and concanavalin A in vitro. This effect also depends
on the duration of hatching. The results of experiments on
simultaneous introduction of this toxin with phosphodiesterase inhibitor
(caffeine) and commercial catalase are indicative of the fact
that the effects from the toxin depend on cyclic adenosine monophosphate
content in cells. The apoptosis progresses when the antioxidant
enzyme increase. The extract derived from the Far Eastern
holothurians allows preventing inhibitory activity of this toxin on
binding cell receptors to concanavalin A and inducing apoptosis
and activity of antioxidant enzymes, thus increasing functional activity
of neutrophils.
Keywords
616-002.71-085.324:593.96
About the Authors
Л. Долматова
Тихоокеанский океанологический институт им. В.И. Ильичева ДВО РАН
Russian Federation
Russian Federation
О. Заика
Тихоокеанский океанологический институт им. В.И. Ильичева ДВО РАН
Russian Federation
Russian Federation
Е. Недашковская
НИИ эпидемиологии и микробиологии СО РАМН
Russian Federation
Russian Federation
Н. Тимченко
НИИ эпидемиологии и микробиологии СО РАМН
Russian Federation
Russian Federation
L. S. Dolmatova
Ilichev Pacific Oceanological Institute, FEB RAS
Russian Federation
Russian Federation
O. A. Zaika
Ilichev Pacific Oceanological Institute, FEB RAS
Russian Federation
Russian Federation
E. P. Nedashkovskaya
Research Institute of Epidemiology
and Microbiology, SB RAMS
Russian Federation
Russian Federation
N. F. Timchenko
Research Institute of Epidemiology
and Microbiology, SB RAMS
Russian Federation
Russian Federation
References
1. Долматова Л.С., Ромашина В.В. Особенности изменения активности антиоксидантных ферментов в различных типах лейкоцитов крови у больных хроническим алкоголизмом // Патологическая физиология и экспериментальная терапия. 2003. № 2. C. 17-19.
2. Долматова Л.С., Тимченко Н.Ф., Стасенко Н.Я. Характеристика состава и медико-биологические исследования комплекса биологически активных веществ из дальневосточных видов голотурий // Дальневосточные моря России. Книга 2. Исследование морской экологии и биоресурсов. М.: Наука, 2007. С. 684-694.
3. Сомов Г.П., Покровский В.И., Беседнова Н.Н., Антоненко Ф.Ф. Псевдотуберкулез. М.: Медицина, 2001. 254 с.
4. Тимченко Н.Ф., Недашковская Е.П., Долматова Л.С., Сомова-Исачкова Л.М. Токсины Yersinia pseudotuberculosis. Владивосток: Примполиграфкомбинат, 2004. 219 с.
5. Chakraborty P., Ghosh D., Basu M.K. Modulation of macrophage mannose receptor affects the uptake of virulent and avirulent Leishmania donovani promastigotes // J. Parasitol. 2001. Vol. 87, No. 5. P. 1023-1027.
6. Davis W., Ronae Z., Tew K. Cellular thiols and reactive oxygen species in drug-induced apoptosis // J. Pharmacol. Exp. Ther. 2001. Vol. 296, No. 1. P. 1-6.
7. Komatsu N., Oda T., Muramatsu T. Involvement of both caspaselike proteases and serine proteases in apoptotic cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin // J. Biochem. 1998. Vol. 124. P. 1038-1044.
8. McKenzie A.N.J., Preston T.M. Functional studies on Calliphora vomitoria haemocyte subpopulations defined by lectin staining and density centrifugation // Develop. Comp. Immunol. 1992. Vol. 16, No. 1. P. 19-30.
9. Nakamura A., Imaizumi A., Yanagawa Y. et al. 2 - Adrenoceptor activation inhibits Shiga toxin2-induced apoptosis of renal tubular epithelial cells // Biochem. Pharmacol. 2003. Vol. 66. P. 343-353.
10. Ruckdeschel K. Immunomodulation of macrophages by pathogenic Yersinia species // Arch. Immunol. Ther. Exp.-Warsz. 2002. Vol. 50, No. 2. P.131-137.
11. Thelen M., Dewald B., Baggolini M. Neutrophil sygnal transduction and activaton of the respiratory burst // Physiol. Rev. 1993. Vol. 73, No. 4. P. 797-821.
12. Thiel M., Zourelidis C., Peter K. Die Rolle der polymorphkernigen neutrophilen Leukozyten in der Pathogenese des akuten Lungenversagens (ARDS) // Anaesthesist. 1996. Bd. 45. S. 113-130.
13. Wang L., Lu L. Pathway-specific effect of caffeine on protection against UV irradiation-induced apoptosis in corneal epithelial cells // Investigative Ophthalmology and Visual Science. 2007. Vol. 48. P. 652-660.
Review
For citations:
, , , , Dolmatova L.S., Zaika O.A., Nedashkovskaya E.P., Timchenko N.F. STUDYING MECHANISMS OF APOPTOSIS -MODULATING ACTIVITY OF THERMORESISTANT TOXIN YERSINIA PSEUDOTUBERCULOSIS AND CORRECTIVE EFFECT OF FAR- EASTERN HOLOTHURIANS -DERIVED EXTRACT IN RAT NEUTROPHILS IN VITRO. Pacific Medical Journal. 2010;(3):76-80. (In Russ.)
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