DIFFERENTIATION INHIBITION OF NEUTRAL STEM CELLS VIA BIO-POLYMER MATRIX MATERIALS
Abstract
The authors analyze properties of bio-polymer
matrix materials being partial structurally-functional analogues
of hyaluronic acid-based glycopolymers. Original modified
polysaccharidic compounds had inhibiting effect on the
capability of neutral stem cells to differentiate. To estimate
cell vitality after cultivation on the substrates under study,
the authors have re-cultivated them under the conditions of
induced adhesion and differentiation. All the studied variants
of glycopolymer matrixes have proved the capability of cells to
differentiate and regular grow under the conditions of neuroinduction
substrate that included dimerous forms of IV type
collagen. One of the materials included modified derivatives
of uronic acids has been recommended as promising matrix
capable of inhibiting undesirable spontaneous differentiation
of neutral stem cells during derivation and conservation of their
biomass needed to create artificial implants of nervous tissue.
matrix materials being partial structurally-functional analogues
of hyaluronic acid-based glycopolymers. Original modified
polysaccharidic compounds had inhibiting effect on the
capability of neutral stem cells to differentiate. To estimate
cell vitality after cultivation on the substrates under study,
the authors have re-cultivated them under the conditions of
induced adhesion and differentiation. All the studied variants
of glycopolymer matrixes have proved the capability of cells to
differentiate and regular grow under the conditions of neuroinduction
substrate that included dimerous forms of IV type
collagen. One of the materials included modified derivatives
of uronic acids has been recommended as promising matrix
capable of inhibiting undesirable spontaneous differentiation
of neutral stem cells during derivation and conservation of their
biomass needed to create artificial implants of nervous tissue.
About the Authors
В. Кумейко
Институт биологии моря им. А.В. Жирмунского ДВО РАН; Дальневосточный государственный университет
Russian Federation
Russian Federation
А. Анисимов
Дальневосточный государственный университет
Russian Federation
Russian Federation
А. Щеблыкина
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Russian Federation
Russian Federation
А. Астахова
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Russian Federation
Russian Federation
Н. Зюмченко
Дальневосточный государственный университет
Russian Federation
Russian Federation
Н. Токмакова
Дальневосточный государственный университет
Russian Federation
Russian Federation
И. Кирсанова
Дальневосточный государственный университет
Russian Federation
Russian Federation
А. Анисимова
Дальневосточный государственный университет
Russian Federation
Russian Federation
Е. Демиденко
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Russian Federation
Russian Federation
И. Дюйзен
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Russian Federation
Russian Federation
М. Хотимченко
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Russian Federation
Russian Federation
V. V. Kumeiko
Institute of Marine Biology named after A.V. Zhirmundsky, FEB RAS; Far Eastern National University
Russian Federation
Russian Federation
A. P. Anisimov
Far Eastern National University
Russian Federation
Russian Federation
A. V. Scheblyikina
Institute of Marine Biology named after A.V. Zhirmundsky, FEB
RAS
Russian Federation
Russian Federation
A. A. Astakhova
Institute of Marine Biology named after A.V. Zhirmundsky, FEB
RAS
Russian Federation
Russian Federation
N. E. Zyumchenko
Far Eastern National University
Russian Federation
Russian Federation
N. P. Tokmakova
Far Eastern National University
Russian Federation
Russian Federation
I. A. Kirsanova
Far Eastern National University
Russian Federation
Russian Federation
A. A. Anisimova
Far Eastern National University
Russian Federation
Russian Federation
E. V. Demidenko
Institute of Marine Biology named after A.V. Zhirmundsky, FEB
RAS
Russian Federation
Russian Federation
I. V. Dyuizen
Institute of Marine Biology named after A.V. Zhirmundsky, FEB
RAS
Russian Federation
Russian Federation
Yu. S. Khotimchenko
Institute of Marine Biology named after A.V. Zhirmundsky, FEB
RAS
Russian Federation
Russian Federation
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Review
For citations:
, , , , , , , , , , , Kumeiko V.V., Anisimov A.P., Scheblyikina A.V., Astakhova A.A., Zyumchenko N.E., Tokmakova N.P., Kirsanova I.A., Anisimova A.A., Demidenko E.V., Dyuizen I.V., Khotimchenko Yu.S. DIFFERENTIATION INHIBITION OF NEUTRAL STEM CELLS VIA BIO-POLYMER MATRIX MATERIALS. Pacific Medical Journal. 2010;(2):20-26. (In Russ.)
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