Preview

Тихоокеанский медицинский журнал

Расширенный поиск

ИССЛЕДОВАНИЕ КИСЛОТНОГО ГИДРОЛИЗА ВЫСОКОЭТЕРИФИЦИРОВАННОГО И НИЗКОЭТЕРИФИЦИРОВАННОГО ПЕКТИНОВ

Полный текст:

Аннотация

The paper studies acid hydrolysis of high-etherified
and low-etherified pectins in 0.5МHCl at 90°С. The acid hydrolysis
of high-etherified pectin is characterized by decreasing
extent of etherification and production of low-etherified pectin.
In case of low-etherified pectin hydrolysis, the rate rapidly
decreases during the first 1.5 hours, and then remains almost
unchanged. The first stage includes production of elements enriched
in neutral saccharides; the second stage - that in polygalacturonic
acid. The molecular mass is likely to cause phase
conversion of hydrolysis products from gel into solution is about
10 kDa. There is dependence between fraction composition and
duration of the hydrolysis.

Об авторах

В. В. Ковалев
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Россия


Е. А. Коленченко
Институт биологии моря им. А.В. Жирмунского ДВО РАН
Россия


К. Е. Макарова
Владивостокский государственный медицинский университет
Россия


V. Kovalev
Institute of Marine Biology named after A.V. Zhirmundsky, FEB RAS
Россия


E. Kolenchenko
Institute of Marine Biology named after A.V. Zhirmundsky, FEB RAS
Россия


K. Makarova
Vladivostok State Medical University
Россия


Список литературы

1. Афанасьев С.П., Чирва В.Ю., Кацева Г.Н. и др. Модифи- кация титриметрических методов анализа пектиновых веществ // Химия природных соединений. 1984. № 4. С. 428-431.

2. Оводов Ю.С. Современные представления о пектиновых веществах // Биоорг. химия. 2009. Т. 35, № 3. С. 293-310.

3. Уистлер Р.Л., Вольфрам М.Л. Методы химии углеводов / пер. с англ. под ред. Кочеткова Н.К. М.: Мир, 1967. 512 с.

4. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids // Anal. Biochem. 1973. Vol. 54. P. 484-489.

5. Chauhan D., Li G., Podar K. et. al. A novel carbohydrate-based therapeutic GCS-100 overcomes bortezomib resistance and enhances dexamethasone-induced apoptosis in multiple myeloma cells // Cancer Res. 2005. Vol. 65. P. 8350-8358.

6. Dongowski G., Lorenz A. Unsaturated oligogalacturonic acids are generated by in vitro treatment of pectin with human faecal flora // Carbohydr. Res. 1998. Vol. 314. P. 237-244.

7. Dubois M., Gilles K.A., Hamilton J.K. et al. Colorimetric method for determination of sugars and related substances // Anal. Chem. 1956. Vol. 28, No. 3. P. 350-356.

8. Eliaz I, Hotchkiss A.T, Fishman M.L, Rode D. The effect of modified citrus pectin on urinary excretion of toxic elements // Phytother Res. 2006. Vol. 20. P. 859-864.

9. Glinskii O.V., Huxley V.H., Glinsky G.V. et. al. Mechanical entrapment is insufficient and intercellular adhesion is essential for metastatic cell arrest in distant organs // Neoplasia. 2005. Vol. 7. P. 522-527.

10. Hayashi A., Gillen A.C., Lott J.R. Effects of daily oral administration of quercetin chalcone and modified citrus pectin on implanted colon-25 tumor growth in Balb-c mice // Altern. Med. Rev. 2000. Vol. 5. P. 546-552.

11. Inohara H., Raz A. Effects of natural complex carbohydrate (citrus pectin) on murine melanoma cell properties related to galectin- 3 functions // Glycoconj. J. 1994. Vol. 11. P. 527-532.

12. Johnson K.D., Glinskii O.V., Mossine V.V. et. al. Galectin-3 as a potential therapeutic target in tumors arising from malignant endothelium // Neoplasia. 2007. Vol. 9. P. 662-670.

13. Nangia-Makker P., Hogan V., Honjo Y. et. al. Inhibition of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus pectin // J. Natl. Cancer Inst. 2002. Vol. 94. P. 1854-1862.

14. Oosterveld A., Beldman G., Schols H.A. Voragen A.G.J. Arabinose and ferulic acid rich pectic polysaccharides extracted from sugar beet pulp // Carbohydr. Res. 1996. Vol. 288. P. 143-153.

15. Sathisha U.V., Jayaram S., Harish Nayaka M.A., Dharmesh S.M. Inhibition of galectin-3 mediated cellular interactions by pectic polysaccharides from dietary sources // Glycoconj. J. 2007. Vol. 24. P. 497-507.


Для цитирования:


Ковалев В.В., Коленченко Е.А., Макарова К.Е., ., ., . ИССЛЕДОВАНИЕ КИСЛОТНОГО ГИДРОЛИЗА ВЫСОКОЭТЕРИФИЦИРОВАННОГО И НИЗКОЭТЕРИФИЦИРОВАННОГО ПЕКТИНОВ. Тихоокеанский медицинский журнал. 2010;(2):62-66.

For citation:


., ., ., Kovalev V.V., Kolenchenko E.A., Makarova K.E. STUDYING ACID HYDROLYSIS OF HIGH-ETHERIFIED AND LOW-ETHERIFIED PECTINS. Pacific Medical Journal. 2010;(2):62-66. (In Russ.)

Просмотров: 6


Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.


ISSN 1609-1175 (Print)