Immunomodulating activity of the Far Eastern sea red algae-derived carrageenans
Abstract
Both in clinic and laboratory, the authors have studied
immunomodulating effects of sulphated polysaccharides - carrageenans - derived from the red algae of Gigartinaceae and Tichocarpaceae families (Sea of Japan). In vitro studies allowed to define dependence between immunomodulating activity and polysaccharides structure. Lambda-carrageenan was deemed to have the most pronounced biological effect proved to increase calcium ion concentration in mice lymphocytes, generate active forms of oxygen in macrophages and induce apoptosis in Ehrlich>s carcinoma cells. Kappa and kappa/iota-carrageenans showed higher capability to induce generation of proinflammatory cytokines. There was concentration dependence between cytokine-induc-ing activity and polysaccharide concentration. Clinical testing allowed to note positive effects of carrageenan used in integrated treatment of patients suffering from acute enteric infections on the immune system and hemostatic parameters.
immunomodulating effects of sulphated polysaccharides - carrageenans - derived from the red algae of Gigartinaceae and Tichocarpaceae families (Sea of Japan). In vitro studies allowed to define dependence between immunomodulating activity and polysaccharides structure. Lambda-carrageenan was deemed to have the most pronounced biological effect proved to increase calcium ion concentration in mice lymphocytes, generate active forms of oxygen in macrophages and induce apoptosis in Ehrlich>s carcinoma cells. Kappa and kappa/iota-carrageenans showed higher capability to induce generation of proinflammatory cytokines. There was concentration dependence between cytokine-induc-ing activity and polysaccharide concentration. Clinical testing allowed to note positive effects of carrageenan used in integrated treatment of patients suffering from acute enteric infections on the immune system and hemostatic parameters.
Keywords
УДК615.37:577.114:[615.322:582.273]
About the Authors
I. M. Ermak
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
V. N. Davidova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
D. L. Aminin
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
A. O. Barabanova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
E. V. Sokolova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
R. N. Bogdanovich
Medical Society, FEB RAS
Russian Federation
Russian Federation
A. M. Polyakova
Central Research Centre of Epidemiology
Russian Federation
Russian Federation
T. F. Soloviova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
References
1. Барабанова А.О., Ермак И.М., Глазунов В.П. и др. Сравнительная характеристика каррагинанов, выделенных из вегетативной и репродуктивной форм водоросли Tichocarpus crinitus (Gmel.) Rupr. (Rhodophyta, Tichocarpaceae) // Биохимия. 2005. Т. 70. С. 430-437.
2. Звягинцева Т.Н., Беседнова Н.H., Елякова Л.Л. Структура и иммунотропное действие 1,3; 1,6-β-D-глюканов. Владивосток: Дальнаука, 2002.
3. Хасина Э.И., Сгребнева М.Н., Ермак И.М., Малеев В.В. Влияние каррагинана на неспецифическую резистентность мышей при ЛПС-индуцированной эндотоксемии // ЖМЭИ. 2007. № 2 С. 57-60.
4. Abe Т., Kawamura H, Watanable H. et. al. Liver injury due to sequential activation of natural killer cells and natural killer T cells by carrageenan // J. Hepatol. 2002. Vol. 36, No. 3. P. 614-623.
5. Bienvenu J., Doche C., Gutowski M. et. al. Production of proinflammatory cytokines and cytokines involved in the TH1/TH2 balance is modulated by pentoxifylline // J. Cardiovasc. Pharmacol. 1995. Vol. 25. P. 80-84
6. Bixler H.J. Recent developments in manufacturing and marketing carrageenan. //Hydrobiologia. 1996. Vol. 326/327. P. 35-57.
7. Falshaw R., Richard H., David E. Stevenson. Structural analysis of carrageenans from the red alga, Callophyllis hombroni-ana Mont. К (Kallymeniaceae, Rhodophyta) // Carbohydr. Research: 2005 Vol. 340, No. 6 P. 1149-1158.
8. Kilpatric D.C. Immunological aspects of the potential role of dietary carbohydrates and lectins in human health//Eur. J. Nutr. 1999.Vol. 38. P. 107-117.
9. Tahaye M.,Kaeffer B. Seaweed dietary fibres: structure, physicochemical and biological properties relevant to intestinal physiology // Sciences and Aliments. 1997. Vol. 17. P. 563-584
10. Popov S.V., Popova G .Yu., Ovodova R.G. et. al. Effects of polysaccharides from Silen vulgaris on phagocytes // Int. J. Immu-nopharmacol. 1999. Vol. 21. P. 617-624.
11. Rosha de Souza M.C., Marques С.Т., Guerra Dore C.M. et. al. Antioxidant activities of sulfated polysaccharides from brown and red seaweeds //J. Appl. Phycol. 2007. Vol. 19. P. 153-160
12. Tadeta K., Irifune K., TomonoK. et. al. Potential activity of carrageenan to enhance antibacterial host-defense system in mice //J. Infect. Chemother. 1995. Vol. 1. P. 59-63.
13. Yermak I.M., Kim Yong Hwan, Titlyanov E.A. et. al. Chemical structure and gel properties of carrageenan from algae belonging to the Gigartinaceae and Tichocapaceae, collected from the Russian Pacific coast //J. Appl. Phycol. 1999. Vol. 11. P. 41-48
14. Yermak I.M., Khotimchenko Yu.S. Chemical properties, biological activities and applications of carrageenan from red algae// in Recent Advances in Marine Biotechnology (Fingerman M, Nagabhushanam R. eds). Sci. Publ. Inc. USA-UK. 2003. Vol. 9. P. 207-255.
15. Yermak I.M., Davidova V. N., Gorbach V.I. et. al. Formingand immunological properties of some lipopolysaccharide-chitosan complexes//Biochimie. 2006. Vol. 88. No 1. P. 23-30
Review
For citations:
Ermak I.M., Davidova V.N., Aminin D.L., Barabanova A.O., Sokolova E.V., Bogdanovich R.N., Polyakova A.M., Soloviova T.F. Immunomodulating activity of the Far Eastern sea red algae-derived carrageenans. Pacific Medical Journal. 2009;(3):40-45. (In Russ.)
Views:
285
Email this article 