Early activation of human peripheral blood lymphocytes and monocytes by components of proteobacte-ria Pseudoalteromonas nigrifaciens
Abstract
Applying bicolour flow cytometry technique by FACSCalibur (Becton Dickinson), the authors have determined effects produced by lipopolysaccharide derived from the sea proteobacteria Pseudoalteromonas nigrifaciens and its structural components on the early activation of human mononuclear blood cells. Lipopolysaccharide, O-specific polysaccharide (O-PS) and oligosaccharide cor (Cor) increased the CD69 and CD25 expression on the CD4+, CD8+, CD16+ lymphocytes and on the CD69 expression monocytes. Lipopolysaccharide increased CD95 expression on the CD4+, CD8+, CD16+ cells; OP-S and Cor increased the CD4+ expression. None of the glycopolymers under study induced statistically significant changes in the CD38 expression. Compared to the polysaccharide, O-PS and Cor caused more intense increase of activation marker expression on the monocytes and lymphocytes. To a greater extent, O-PS and Cor activated cells capable of producing cytotoxic effect.
Keywords
УДК612.112:612.017.1:579.841.11:577.114
About the Authors
Т. Р. Smolina
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
T. S. Zaporozhets
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
R. P. Gorshkova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
E. L. Nazarenko
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
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Review
For citations:
Smolina Т.Р., Zaporozhets T.S., Gorshkova R.P., Nazarenko E.L. Early activation of human peripheral blood lymphocytes and monocytes by components of proteobacte-ria Pseudoalteromonas nigrifaciens. Pacific Medical Journal. 2009;(3):45-48. (In Russ.)
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