Early activation of human peripheral blood lymphocytes and monocytes by components of proteobacte-ria Pseudoalteromonas nigrifaciens
Abstract
Applying bicolour flow cytometry technique by FACSCalibur (Becton Dickinson), the authors have determined effects produced by lipopolysaccharide derived from the sea proteobacteria Pseudoalteromonas nigrifaciens and its structural components on the early activation of human mononuclear blood cells. Lipopolysaccharide, O-specific polysaccharide (O-PS) and oligosaccharide cor (Cor) increased the CD69 and CD25 expression on the CD4+, CD8+, CD16+ lymphocytes and on the CD69 expression monocytes. Lipopolysaccharide increased CD95 expression on the CD4+, CD8+, CD16+ cells; OP-S and Cor increased the CD4+ expression. None of the glycopolymers under study induced statistically significant changes in the CD38 expression. Compared to the polysaccharide, O-PS and Cor caused more intense increase of activation marker expression on the monocytes and lymphocytes. To a greater extent, O-PS and Cor activated cells capable of producing cytotoxic effect.
Keywords
УДК612.112:612.017.1:579.841.11:577.114
About the Authors
Т. Р. Smolina
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
T. S. Zaporozhets
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
R. P. Gorshkova
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
E. L. Nazarenko
Pacific Institute of Bioorganic Chemistry, FEB RAS
Russian Federation
Russian Federation
References
1. Горшкова Н.М. Таксономические критерии в систематике морских аэробных протеобактерий: автореф. дис. ... кан. биол. наук. Владивосток. 2000. 26 с.
2. Горшкова Р.П., Назаренко Е.Л., Зубков А.А. и др. Структура повторяющегося звена кислого полисахарида Alteromonas haloplanktis KMM156// Биоорган, химия. 1993. Т. 19, № 3. С. 327-336.
3. Смолин В.А. Математическое моделирование в геологии и геофизике (Статистика). Владивосток: ДВГТУ. 2007. 232 с.
4. Смолина Т.П., Горшкова Р.П., Назаренко Е.Л. и др. Блокирование адгезии Yersinia pseudotuberculosis с помощью полисахаридов, выделенных из морских микроорганизмов Pseudoal-teromonas // Тихоокеанский мед. журн. 2001. № 2. С. 18-20.
5. Смолина Т.П., Горшкова Р.П., Назаренко Е.Л. и др. Ингибирование адгезии прокариотических и эукариотических клеток липополисахаридом и его фрагментами из морских протеобактерий Pseudoalteromonas nigrifaciens KMM156 //Антибиотики и химиотерапия. 2005. №5-6. С.4-6.
6. Clausen J., Vergeiner В., Enk M. et al. Functional significance of the activation-associated receptor CD25 and CD69 on human NK-cells and NK-like T-cells//Immunobiology. 2003. V. 207, No. 2. P. 85-93.
7. De Maria R., Cifone M.G., R. Trotta R et al. Triggering of human monocyte activation through CD69, a member of the natural killer cell gene complex family of signal transducing receptors//Exp. Med. 1994. Vol. 180. P. 1999-2004.
8. Ghiringhelli F., Menard C., Terme M. et al. CD4+CD25+ T-regulatory cells inhibit natural killer cell functions in a transforming growth factor-β-dependent manner // Exp. Med. 2005. Vol.202, No. 8 P. 1075-1085
9. Goodier M.R., Eondei M. Lipopolysaccharide stimulates the proliferation of human CD56+CD3 NK cells: a regulatory role of monocytes and IL10//Immunology. 2000 Vol. 165, No. 1. P. 139-147
10. Marzio R, Mauel J., Betz-Corradin S. CD69 and regulation of the immune function // Immunopharmacol Immunotoxicol. 1999. Vol. 21, No. 3. P. 565-582.
11. Mattern Т., Flad H., Brade L. et al. Stimulation of human T-lymphocytes by LPS is MHC unrestricted but strongly dependent on B7 interaction//Immunology. 1998. Vol. 160. P. 3412-3418.
12. Sandoval-Montes C, Santos-Argumedo L. CD38 is expressed selectively during the activation of a subset of mature T-cells with redused proliferation but improved potential to produce cytokines // Leukocyte Biology. 2005. Vol. 77. P. 513-521.
13. Trzonkowski P.E., Szynit J., Mysliwska A. et al. CD4+CD25+ T regulatory cells inhibit cytotoxic activity of T CD8+ and NK lymphocytes in the direct cell-to-cell interaction // Clin. Immunol. 2004. Vol. 112. P. 258-267.
14. Vilanova M., Tavares. D., Ferreira P. et al. Role of Monocytes in the Up-Regulation of the Early Activation Marker CD69 on В and T Murine Lymphocytes Induced by Microbial Mitogens Scandinavian // Immunology. 1996. Vol. 43, No. 2. P. 155-163.
Review
For citations:
Smolina Т.Р., Zaporozhets T.S., Gorshkova R.P., Nazarenko E.L. Early activation of human peripheral blood lymphocytes and monocytes by components of proteobacte-ria Pseudoalteromonas nigrifaciens. Pacific Medical Journal. 2009;(3):45-48. (In Russ.)
Views:
222
Email this article 