Changing metabolism of phagocytes in blood in response to action of salmon milt-derived deoxyribonu-cleic acid
Abstract
The paper provides an integrated study into the state of oxygen-dependent systems of phagocytes in blood. As shown, there was a dose-dependent stimulating effect produced by DNA on these cells that appeared to have capability of decreasing activity of ferments that participated in generation of superoxide anionic radical and, in contrast, increasing activity of myeloperoxidase being a catalyst of oxygen anion conversion into hydrogen peroxide. This allowed to consider the salmon milt DNA to have antioxidative effect with respect to hemato-phages. Besides, stimulatory effects of this substance on other phagocyte functions (intracellular contents of 5'-nucleotidases and cationic proteins) could be determinative for prospects of applying the salmon milt-derived DNA as immune-response stimulating drug because the cationic proteins have been known to be representatives of low-molecular intracellular regulators very likely to participate in signalling various physiological functions of an organism.
Keywords
УДК612.112:612.017.1:577.213/217:[615.324:597.552.51]
About the Authors
N. G. Plekhova
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
L. N. Fedyanina
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
L. M. Somova
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
N. N. Besednova
Research Centre of Epidemiology and Microbiology of the RAMS, Siberian Branch
Russian Federation
Russian Federation
Т. К. Kalenik
Pacific State University of Economics
Russian Federation
Russian Federation
References
1. Клебанов Г.И., Владимиров Ю.А. Клеточные механизмы прайминга и активации фагоцитов // Успехи совр. биол. 1999. Т. 119, № 5. С. 462-475.
2. Кольман Я., Рем К. -Т. Наглядная биохимия. М.: Мир, 2000. 468 с.
3. Митькин В.В., Волков Н.И., Пшеничникова Т.Я., Сухих Т.Г. Действие даназола на активность 5'-нуклеатидазы перитонеальных макрофагов мышей// Бюл. экп. биол. имед.1992№2.С.51-54
4. Нестерова И.В. Вторичные иммунодефицитные состояния: справочник по иммунологии. СПб.: Диалог, 2002. 214с.
5. Нестерова И.В., Сепиашвили Р.И. Иммунотропные препараты и современная иммунотерапия в клинической иммунологии и медицине//Иммунология. 2002. Т. 23, № 3. С. 132-138.
6. Определение функциональной активности лейкоцитов периферической крови в качестве показателя неспецифической защиты организма: методические рекомендации / Сомова Л.М., Плехова Н.Г., Кондрашова Н.М., Запорожец Т.С Владивосток: Полиграфкомбинат, 2005. 24 с.
7. Способ получения ДНК из молок рыб/ Гаймула М.А., Кална В.Х., Микстайс У.Я., Эпштейн Л.М. Пат. СССР №915446. Заяв. 10.10.1980; опубл. 01.07.1991г.
8. Тотолян А.А., Фрейдлин И.С. Клетки иммунной системы. СПб: Наука, 2000. 232 с.
9. Шамова О.В., Сакута Г.А., Орлов Д.С. и др. Действие антимикробных пептидов из нейтрофильных гранулоцитов на опухолевые и нормальные клетки в культуре // Цитология. 2007. Т. 49, № 12. С. 1000-1010.
10. Ваbiol В. М. NADPH oxidase: an update // Blood. 1999. Vol. 93, No. 5 P. 1464-1476.
11. Ferencik M., Stefanovic J. Lysosomal enzymes of phagocytes and the mechanism of their release // Folia microbial. Praha. 1999. Vol. 24, No. 6 P. 503-575
12. Forman H.J., Torres M. Reactive oxygen species and cell signaling (Respiratory burst in macrophage signaling) //Am. J. Respir Crit. Care Med. 2002. Vol. 166. P. S4-S8.
13. Levy О. Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes // J. Leukocyte Biology. 2004. Vol. 74. P. 909-925.
14. Livescu A., Manda G., Constantin C. et al. Plasma membrane potential interferes with the respiratory burst of peripheral granulocytes//J. Cell. Mol. Med. 2003. Vol. 7, No. 1. P. 73-78.
Review
For citations:
Plekhova N.G., Fedyanina L.N., Somova L.M., Besednova N.N., Kalenik Т.К. Changing metabolism of phagocytes in blood in response to action of salmon milt-derived deoxyribonu-cleic acid. Pacific Medical Journal. 2009;(3):55-58. (In Russ.)
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