Indication of the hospital strains of Pseudomonas aeruginosa by the method of the restriction analysis of polimorphism of genes
Abstract
The 27 strains of the Pseudomonas aeruginosa by the analysis of polymorphism of lengths of the restricted DNA fragments in polymerase chain reaction with application of the endonucleases of the restriction were investigated. The most convenient for typing of the closely related strains of pseudomonads appeared the restriction gene ExoY by the enzyme Sal I. It allows recommending it for the revealing of a source and establishing of the mechanisms of infection transmission.
About the Authors
V. Turkutyukov
Vladivostok State Medical University
Russian Federation
Russian Federation
L. Balabanova
Pacific Institute of Bioorganic Chemistry of the Far East branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
E. Slabenko
Vladivostok State Medical University
Russian Federation
Russian Federation
References
1. Гинцбург А.Л., Ильина Т.С., Романова Ю.М. QUORUM SENSING социальное поведение бактерий // Журн. микробиол., эпидемиол., иммунол. 2003. № 5. С. 86-93.
2. Гинцбург А.Л., Шагинян И.А. Роль молекулярно-генетических технологий в повышении качества диагностики инфекционных заболеваний // Внутриутробные инфекции плода и новорожденного: мат. Всерос. конф. Саратов, 2000. С. 47-49.
3. Гланц С. Медико-биологическая статистика / пер. с англ. М.: Практика, 1998. 460 с.
4. Шагинян И.А., Гинцбург А.Л. ПЦР-генетическое типирование возбудителей бактериальных инфекций // Генетика. 1995. № 31. С. 600-610.
5. Tamm M., Todisco T., Feldman C. et al. Clinical and bacteriological outcomes in hospitalised patients with community-acquired pneumonia treated with azithromycin plus ceftriaxone, or ceftriaxone plus clarithromycin or erythromycin: a prospective, randomised, multicentre study // Clin. Microbiol. Infect. 2007. Vol. 13, No. 2. P. 162-171.
Review
For citations:
Turkutyukov V., Balabanova L., Slabenko E. Indication of the hospital strains of Pseudomonas aeruginosa by the method of the restriction analysis of polimorphism of genes . Pacific Medical Journal. 2009;(1):59-61. (In Russ.)
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