Results of the effect of toluene vapors on the reproductive system of male rats
https://doi.org/10.17238/PmJ1609-1175.2017.3.54-58
Abstract
The study objective is to analyze changes in the internal organs of the male reproductive system of mature rats after inhalation exposure to toluene, a component of epoxy resins widely used in industry. Methods. The experiment was performed on 60 adult male white rats. 30 animals were exposed to inhalation exposure to toluene at a concentration of 500 mg/m3 for 60 days (5 days a week for 5 hours per day). 30 intact rats were used as a control group. Groups of animals (6 rats each) were withdrawn from the experiment on the 1st, 7th, 15th, 30th and 60th days after the termination of exposure to the toxicant. Histological and histomorphometric examination of internal genital organs was performed. Results. Under conditions toluene effect on the human body, disintegration of germinal epithelium of testes, mild vacuolation of Leydig and Sertoli cells, insignificant decrease in spermatogenesis index, decrease in secretion in the prostate gland and a moderate decrease in the height of the epithelium of seminal vesicles were observed. Conclusions. The study for the first time describes the effect of chemical exposure of toluene on the entire complex of organs of the reproductive system of rats. The question of whether the data obtained can be compared with the results of similar studies requires a separate analysis.
References
1. Евразийский совет по стандартизации, метрологии и сертификации (ЕАСС). Методы испытания по воздействию химической продукции на организм человека. Испытания по оценке репродуктивной/эмбриональной токсичности (скрининговый метод). М.: Стандартинформ, 2013. 18 с
2. Каликин К.Г., Высоцкий И.Ю., Гречишкина Т.Ф., Сиротина М.О. Состояние перекисного окисления липидов в организме животных при воздействии летучих компонентов эпоксидных смол // Український медичний альманах. 2008. Т. 11, № 6 (додаток). С. 136
3. Мишалов В.Д. Чайковский И.В., Твердохлеб Ю.Б. О правовых, законодательных и этических нормах и условиях при выполнении научных морфологических исследований // Морфология. 2007. Т. 1, № 2. С. 108-115
4. Теплова Т.Е., Богатырева Е.В., Ли Я.Б. [и др.]. К вопросу о нормировании модифицированной эпоксидной смолы марки уп-666-4 в воздухе рабочей зоны // Актуальные проблемы транспортной медицины. 2005. № 2. С. 84-88
5. Федченко С.Н, Галузина Л.О. Структурные особенности стенки желудка крыс при хронической ингаляции толуолом // Перспективи медицини та біології. 2010. Т. 2, № 1 (додаток). С. 75
6. Aitken R.J., Roman S.D. Antioxidant systems and oxidative stress in the testes // Oxidative Medicine and Cellular Longevity. 2008. No. 1. Р 115-124.
7. Aktas C., Kanter M., Erboga M. Anti-apoptotic effects of curcumin on cadmium-induced apoptosis in rat testis // Toxicology and Industrial Health. 2011. Vol. 28, No. 2. Р 122-130.
8. Cheng C.Y., Wang Q., Wang F.F. Stress induces glucocorticoid-mediated apoptosis of rat Leydig cells in vivo // Stress. 2012. Vol. 15, No. 1. P 74-78.
9. El-Shahat A.E., Gabr A., Meki A.R., Mehana E.S. Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract // International Journal of Morphology. 2009. Vol. 27, No. 3. P. 757-764.
10. European convention for the protection of vertebrate animals used for experim. and other scientific purposes. Coun. Of Europe. Strasbourg, 1986. 53 p.
11. Hsieh G.C., Parker R.D.R., Sharma R.P Subclinical effects of ground water contaminants. III. Effects of repeated oral exposure to combinations of benzene and toluene on immunologic responses in mice // Archive Toxicology. 1990. No. 64. Р 320-328.
12. McWilliams M.L., Chen G.D., Fechter L.D. Low-level toluene disrupts auditory function in guinea pigs // Toxicology and Applied Pharmacology. 2000. No. 167. Р 18-29.
13. Ono A., Sekita K., Ogawa Y. Reproductive and developmental toxicity studies of toluene. II. Effects of inhalation exposure on fertility in rats // Journal of Environmental Pathology, Toxicology and Oncology. 1996. Vol. 15. P. 9-20.
14. Poon R., Chu I.H., Bjarnason S. Inhalation toxicity study of methanol, toluene, and methanol/toluene mixtures in rats: effects of 28-day exposure // Toxicology Industrial Health. 1994. No. 10. Р 231-245.
15. Von Euler, Pham T.M., Hillefors M. Inhalation of low concentrations of toluene induces persistent effects on a learning retention task, beam-walk performance, and cerebrocortical size in the rat // Experimental Neurology. 2000. No. 163. Р 1-8.
Review
For citations:
Voloshina I.S. Results of the effect of toluene vapors on the reproductive system of male rats. Pacific Medical Journal. 2017;(3):54-57. (In Russ.) https://doi.org/10.17238/PmJ1609-1175.2017.3.54-58
Views:
288
Email this article 