Elaboration of the method of cultivation of the limbic epithelial stem cells of the cornea
https://doi.org/10.17238/PmJ1609-1175.2017.3.73-74
Abstract
To analyze the possibility of culturing limbal epithelial stem cells, an experiment was performed on rabbits of the Chinchilla breed. Cultivation of cells obtained from fragments of limb tissue was performed on aMEM nutrient environment. On the 8th day, the growth of colonies of epithelial-like and fibroblastlike cells was noted. After treatment with antibodies against cy-tokeratin 19, the presence of limbal epithelial stem cells in the grown population was confirmed.
About the Authors
A. S. Dubovikov
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
A. V. Konkieva
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
A. N. Kulikov
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
S. V. Churashov
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
V. F. Chernysh
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
M. I. Blinova
Institute of Cytology ofthe Russian Academy of Science
Russian Federation
Russian Federation
O. I. Aleksandrova
Military Medical Academy named after S.M. Kirov
Russian Federation
Russian Federation
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Review
For citations:
Dubovikov A.S., Konkieva A.V., Kulikov A.N., Churashov S.V., Chernysh V.F., Blinova M.I., Aleksandrova O.I. Elaboration of the method of cultivation of the limbic epithelial stem cells of the cornea. Pacific Medical Journal. 2017;(3):73-74. (In Russ.) https://doi.org/10.17238/PmJ1609-1175.2017.3.73-74
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