Вирусные векторы в трансгенных исследованиях: перспективы использования для лечения болезней ЦНС и генной терапии
https://doi.org/10.34215/1609-1175-2022-1-46-55
Аннотация
Вирусные переносчики представляют большой клинический интерес из-за их высокой эффективности, позволяющей находить практическое применение в генной терапии. В обзоре приводятся современные данные исследований на различных видах рыб в качестве потенциальных моделей для использования вирусных векторов. В ходе изучения высокопроизводительных аденовирусных векторов получены данные об устойчивой экспрессии трансгенов в организме Danio rerio и других видов рыб. Рассмотрены особенности применения аденоассоциированных векторов в исследовании транснейронального переноса белка глюкоронидазы в проекционных нейронах вентральной тегментальной области и стриатума для лечения метаболической недостаточности при мукополисахаридозе VII. Принимая во внимание широкий репертуар генетически модифицированных линий Danio rerio, этические аспекты и допустимость этой модели в нейрогеннных исследованиях, очевидно, что Danio rerio, как и другие виды рыб, могут быть альтернативой для ранней стадии доклинической оценки эффективности вирусных векторов.
Ключевые слова
аденовирус,
генный таргетинг,
Danio rerio,
генная терапия,
вентральная тегментальная область,
глюкоронидаза
При поддержке: Источник финансирования: работа выполнена при финансовой поддержке гранта РФФИ в рамках научного проекта № 20-34-90091
Об авторах
Е. В. Пущина
Национальный научный центр морской биологии им. А.В. Жирмунского, Дальневосточное отделение Российской академии
Россия
Россия
Пущина Евгения Владиславовна – доктор биологических наук, профессор РАН, главный научный сотрудник лаборатории Клеточной дифференциации.
690041, Владивосток, ул. Пальчевского, 17; тел.: +79990400751
И. А. Капустянов
Национальный научный центр морской биологии им. А.В. Жирмунского, Дальневосточное отделение Российской академии
Россия
Россия
Капустянов Илья Александрович - аспирант лаборатории Клеточной дифференциации.
690041, Владивосток, ул. Пальчевского, 17, тел.: +79020521267
А. А. Вараксин
Национальный научный центр морской биологии им. А.В. Жирмунского, Дальневосточное отделение Российской академии
Россия
Россия
Вараксин Анатолий Алексеевич - доктор биологических наук, профессор, ведущий научный сотрудник лаборатории Клеточной дифференциации
690041, Владивосток, ул. Пальчевского, 17, тел.: +79146723560
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Рецензия
Для цитирования:
Пущина Е.В., Капустянов И.А., Вараксин А.А. Вирусные векторы в трансгенных исследованиях: перспективы использования для лечения болезней ЦНС и генной терапии. Тихоокеанский медицинский журнал. 2022;(1):46-55. https://doi.org/10.34215/1609-1175-2022-1-46-55
For citation:
Pushchina E.V., Kapustyanov I.A., Varaksin A.A. Viral Vectors in Transgenic Research: Prospects for the Treatment of CNS Diseases and Gene Therapy. Pacific Medical Journal. 2022;(1):46-55. (In Russ.) https://doi.org/10.34215/1609-1175-2022-1-46-55
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