Fucoidan from the tang Fucus evanescens: immunophenotypic and morphological changes of dendritic cells - effectors of innate immunity
https://doi.org/10.17238/PmJ1609-1175.2018.4.75-79
Abstract
Objective. The study of the mechanism of action of sulfated polysaccharide from the tang Fucus evanescens on the morphological and immunophenotypic characteristics of dendritic cells (DC) -the key effectors of innate immunity - will enable to establish a rational spectrum for its use. Methods. We investigated a partially acylated sulfated fucoidan from the tang F. evanescens. Immature DCs were obtained from bone marrow cells of BALB/c mice. Fucoidan (100 μg/ml) and commercial TNFα (20 ng/ml) were added as maturation inducers. Immunophenotypic cells were determined by flow cytometry using monoclonal antibodies. Results. Fucoidan induced the maturation of DC, as evidenced by their immunophenotypic and morphological changes. A pool of mature cells capable of presenting the antigen to naive T-lymphocytes and activating the effector mechanisms of innate immunity was formed. Conclusions. Thus, the sulfated polysaccharide from F. evanescens, which contributes to the activation of effector cells of innate immunity, can be used as a potential adjuvant to create an anti-infective defense of the body.
About the Authors
I. D. Makarenkova
Somov Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
S. P. Ermakova
G.B. Elyakov Pacific Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
N. K. Akhmatova
Mechnikov Scientific Research Institute of Vaccines and Serums
Russian Federation
Russian Federation
T. I. Imbs
G.B. Elyakov Pacific Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
I. B. Semenova
Mechnikov Scientific Research Institute of Vaccines and Serums
Russian Federation
Russian Federation
M. Yu. Khotimchenko
Far Eastern Federal University
Russian Federation
Russian Federation
N. N. Besednova
Somov Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
M. A. Makarenkov
Medical Association of the Far Eastern Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
T. N. Zvyagintseva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
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Review
For citations:
Makarenkova I.D., Ermakova S.P., Akhmatova N.K., Imbs T.I., Semenova I.B., Khotimchenko M.Yu., Besednova N.N., Makarenkov M.A., Zvyagintseva T.N. Fucoidan from the tang Fucus evanescens: immunophenotypic and morphological changes of dendritic cells - effectors of innate immunity. Pacific Medical Journal. 2018;(4):75-78. (In Russ.) https://doi.org/10.17238/PmJ1609-1175.2018.4.75-79
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