Epidemiology of pneumococcal serotypes in children with outpatient pneumonia and carriers in Krasnoyarsk
https://doi.org/10.34215/1609-1175-2019-4-78-82
Abstract
Objective: The objective is to analyze the seroepidemiology and resistance of Streptococcus pneumoniae in children over 5 y.o.
Methods: 57 patients with pneumonia and 186 healthy children were examined. The induced sputum and nasopharyngeal swabs were analyzed with culture and molecular assays.
Results: S. pneumoniae incidence among healthy children was 15.1 %. Serotype flora was represented by 4 serotypes and 6 serogroups with prevalence of 19F, 37 (for 17.8 % each), 6АВС, 3 (for 10.7 % each). 17.8 % of strains were antibiotic resistant, four of them was 19F serotype, and one was of the 6th serogroup. All of them were multiresistant: to macrolides, lincosamides, tetracyclines, cephalosporins of II generation along with moderate resistance to penicillin. In children with pneumonia, pneumococcocus was detected in 24.6 % of cases: serotypes 19F (64.3 %), 19А (21.4 %), 23F (14.3 %). 57.1 % of strains were multiresistant and was of 19F and 19А serotypes. Phenotype of the resistance was identic to the described above except the resistance to amoxicillin: all strains were resistant to it. All given isolates were moderately resistant to ceftriaxone.
Conclusions: Serotype flora of pneumococci in healthy children of school age was distinct in significant variety with high incidence of ‘mucoid’ serotypes 3 and 37 (28.6 %). In pneumonia, pneumococci of 19F and 19A types occurred significantly frequently. The main resistant serotype of S. pneumoniae in both groups was 19F with lowered sensitivity to beta-lactam antibiotics.
About the Authors
I. N. Protasova
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Irina N. Protasova, MD, PhD, associate professor, Microbiology Department
1 Partizana Zheleznyaka St., Krasnoyarsk, 660022
N. A. Ilyenkova
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Russian Federation
Krasnoyarsk
Russian Federation
Krasnoyarsk
E. S. Sokolovskaya
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Russian Federation
Krasnoyarsk
Russian Federation
Krasnoyarsk
References
1. Community-acquired pneumonia in children. Clinical guidelines. Moscow: OriginalMaket; 2015. 64 p. (In Russ.).
2. Pneumonia: WHO Fact Sheet (November 7, 2016). URL: http://www.who.int/ru/news-room/fact-sheets/detail/pneumonia (Accessed: December 6, 2018).
3. Children: Reducing mortality. WHO Fact Sheet (October 31, 2017). URL: http://www.who.int/ru/news-room/fact-sheets/detail/pneumonia (Accessed: December 6, 2018).
4. Geppe NA, Malakhov AB. Pneumococcal infection of the respiratory system in childhood. Practical guidelines for doctors. Moscow; 2005. 84 p. (In Russ.).
5. Kozlov RS. Pneumococci: Lessons from the past – a look into the future. Smolensk; 2010. 128 p. (In Russ.).
6. Samsygina GA. Infections of the respiratory tract in young children. Moscow: Miklosh; 2006. 279 p. (In Russ.).
7. Strategy and tactics of rational use of antimicrobial agents in outpatient practice: Russian practical recommendations. Eds: Yakovleva SV, Sidorenko SV, Rafalskiy VV, Spichak ТВ. Moscow: Presto Publishing House; 2014. 121 p. (In Russ.).
8. Ferreira M, Oliveira H, Costa e Silva N, Januario L, Rodrigues F. Paediatric invasive pneumococcal disease before universal vaccination: 1995–2015. Acta Med Port. 2017;30(6):457–62.
9. Mayanskiy N, Alyabieva N, Ponomarenko O, Lazareva A, Katosova L, Ivanenko A, et al. Serotypes and antibiotic resistance of non-invasive Streptococcus pneumoniae circulating in pediatric hospitals in Moscow, Russia. Int J Infect Dis. 2014;20:58–62.
10. Verstrheim DF, Hoiby EA, Aaberge IS, Caugant DA. Phenotypic and genotypic characterization of Streptococcus pneumoniae strains colonizing children attending day-care centers in Norway. J Clin Microbiol. 2008;46:2508–18.
11. Webster J, Theodoratou E, Nair H, Ang C, Zgaga L, Huda T, et al. An evaluation of emerging vaccines for childhood pneumococcal pneumonia // BMC Public Health. 2011;11(Suppl 3):S26.
12. PCR deduction of pneumococcal serotypes. URL: https://www.cdc.gov/streplab/pcr.html (Accessed: October 6, 2018).
13. EUCAST. Clinical breakpoints – bacteria (2017). URL: http://www.eucast.org/clinical_breakpoints/ (Accessed: November 6, 2018).
14. Sheppard CL., Kapatai G, Broughton K, Schaefer U, Hannah M, Litt DJ, Fry NK. Clinical streptococcal isolates, distinct from Streptococcus pneumoniae, but containing the β-glucosyltransferase tts gene and expressing serotype 37 capsular polysaccharide. PeerJ. 2017;5:e3571.
Review
For citations:
Protasova I.N., Ilyenkova N.A., Sokolovskaya E.S. Epidemiology of pneumococcal serotypes in children with outpatient pneumonia and carriers in Krasnoyarsk. Pacific Medical Journal. 2019;(4):78-82. (In Russ.) https://doi.org/10.34215/1609-1175-2019-4-78-82
Views:
622
Email this article 