Expression of apoptosis and inflammation markers in skin of rats in the treatment of experimental thermal burns with nanosilver and its combination with a proteinase inhibitor
https://doi.org/10.34215/1609-1175-2020-2-56-60
Abstract
Objective: To substantiate a possibility for modification of inflammation process in thermal burns of stage II using nanoparticles of silver and proteinase inhibitors. Methods: The experiment included 30 mature Wistar male rats. After a thermal burn simualtion, 10 animals were locally applied with a water-soluble composition of silver nanoparticles in a sodium alginate matrix, 10 – with a combination of a water-soluble composition of nanosilver and aprotinin (another 10 rats were control). After 7 days, animals were withdrawn from the experiment and were run histological and immunohistochemical examination of the skin in the burn area. Results: The maximum effect of combined wound therapy with a water-soluble composition of silver nanoparticles in a matrix of sodium alginate with a proteinase inhibitor (aprotinin), which contributed to the activation of regenerative processes, was revealed. Conclusions: The effect of combined therapy is related to an improvement in the balance of proliferation and apoptosis in a burn wound as well as with a specific stimulation of the inflammatory response. This local therapy for burns can be considered pathogenically justified and promising.
About the Authors
O. A. Pisareva
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Russian Federation
Olga A. Pisareva, MD, postgraduate student, Department of General and Clinical Pathophysiology
5/7 Lenina Blvd, Simferopol
A. V. Kubyshkin
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Simferopol
Russian Federation
Simferopol
E. Yu. Bessalova
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Simferopol
Russian Federation
Simferopol
T. P. Makalish
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Simferopol
Russian Federation
Simferopol
I. I. Fomochkina
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Simferopol
Russian Federation
Simferopol
D. V. Chegodar
Medical Academy named after S.I. Georgievsky, Vernadsky Crimean Federal University
Russian Federation
Simferopol
Russian Federation
Simferopol
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Review
For citations:
Pisareva O.A., Kubyshkin A.V., Bessalova E.Yu., Makalish T.P., Fomochkina I.I., Chegodar D.V. Expression of apoptosis and inflammation markers in skin of rats in the treatment of experimental thermal burns with nanosilver and its combination with a proteinase inhibitor. Pacific Medical Journal. 2020;(2):56-60. (In Russ.) https://doi.org/10.34215/1609-1175-2020-2-56-60
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