Microcrystography as standard method of saliva assessment

Abstract

Objective: Standardization of microcrystallization method as a basis for studying saliva parameters affected by various factors. Methods: An improvement of the oral fluid crystallography technique is proposed. 108 children aged 7–12 years were examined. The prevalence and intensity of caries, the degree of activity and growth of caries, and the simplified hygiene index were determined. Mixed saliva samples were taken according to a standardized microcrystallography technique in its own modification. Results: The proportion of high-quality samples of droplets and facies of saliva as a whole was 98 %. During orthodontic treatment, changes in the state of the oral cavity and oral fluid were revealed: a deterioration in the level of hygiene in children with removable orthodontic equipment with an increase in the frequency of occurrence of types II and III saliva microcrystallization (SMC) was almost doubled. After orthodontic treatment, there was a recovery of hygienic parameters and the recorded frequency of I type SMC was increased. Conclusions: A change in the type of SMC depends on a number of factors: oral hygiene, the degree of caries activity and the presence of its progression. A significant increase in the frequency of registration of type III SMC during orthodontic treatment demonstrates a higher likelihood of occurrence and the decompensated nature of the carious process, increases the risk of inflammatory and allergic reactions from the tissues of the oral cavity.