The spatial organization of gas-transmitral neurons in the brain
Abstract
Background. The research objective is an analysis of spatial relations of gas-transmitral neurons in the caudal portion of the brain stem of rats. Methods. The study was performed on 12 adult male rats Wistar weighing 200-240g. Immunopositive neurons groups were studied with immunohistochemical methods for nitric oxide synthase, heme oxygen-ase-2, and cystathionine β-synthase in the projection of the nucleus of the solitary tract, small-cell and gi-ant cell reticular nucleus. Results. Dimensions, share and concentration of NO-, CO- and H2S-of immunopositive neurons as well as their distribution in each of the investigated nuclei are exceptionally unique. Most of these cells were located in close proximity to each other, forming more or less distinct clusters of neurons of different me-diator specificity. conclusions. In the organization of vasomotor nuclei there are structural conditions for cooperation NO-, CO- and H2S- of immunopositive neurons and their integration into functional nerve centers of the brain that enhances the management of complex processes in the body.
About the Authors
M. S. Startseva
Pacific State Medical University
Russian Federation
Russian Federation
A. E. Kotsuba
Pacific State Medical University
Russian Federation
Russian Federation
V. M. Chertok
Pacific State Medical University
Russian Federation
Russian Federation
References
1. Коцюба А.Е., Кокошина В.В., Черток В.М. Возрастные преобразования нитроксидергических нейронов в ядре солитарного тракта у крыс // Тихоокеанский мед. журн. 2009. № 4. C. 50-54.
2. Коцюба А.Е., Черток В.М. Пространственная организация серотонинергических и нитроксидергических нейронов в некоторых ядрах бульбарного отдела сердечно-сосудис того центра человека // Тихоокеанский мед. журнал. 2010. № 4. C. 43-46.
3. Черток В.М., Коцюба А.Е., Беспалова Е.П. Роль оксида азота в реакции артериальных сосудов на лазерное облучение // Бюллетень экспериментальной биологии и медицины. 2008. Т. 145, № 6. С. 699-703.
4. Черток В.М., Коцюба А.Е. Особенности распределения ферментов синтеза H2S в стенке церебральных артерий у крыс // Бюлл. эксперим. биол. и мед. 2012. № 7. C. 116-120.
5. Черток В.М., Коцюба А.Е. Новые нейротрансмиттеры и их роль в центральных механизмах регуляции кровообращения // Тихоокеанский мед. журн. 2013. № 4. C. 27-38.
6. Черток В.М., Коцюба А.Е. Норадреналинергические и нитроксидергические нейроны «вазомоторных» ядер у гипертензивных крыс // Бюлл. эксперим. биол. и мед. 2014. Т. 158, № 11. C. 649-654.
7. Черток В.М., Коцюба А.Е., Старцева М.С. Применение метода компьютерного совмещения изображений для топохимического картирования нейронов мозга // Тихоокеанский мед. журнал. 2014. № 3. C. 77-79.
8. Hogg N. Nitric oxide expands scope to cover hydrogen sulfide and carbon monoxide // Nitric Oxide. 2013. Vol. 35. Р 1-3.
9. Hovater M.B., Ying W.-Zh., Agarwal A., Sanders PW. Nitric oxide and carbon monoxide antagonize TGF-β through ligandindependent internalization of TβR1/ALK5 // Am. J. Physiol. Renal. Physiol. 2014 Vol. 307, No. 6. Р 727-735.
10. Kajimura M., Fukuda R., Bateman R.M. [et al.]. Interactions of multiple gas-transducing systems: hallmarks and uncertainties of CO, NO, and H2S gas biology // Antioxid Redox Signal., 2010. Vol. 13, No. 2. P 157-192.
11. Kotsiuba A.E., Chertok V.M. Immunolocation of cystationin β-syntase in cerebral pontine nuclei in humans // Bull. Exper. Biol. a. Med. 2013. Vol. 155, No. 2. P 277-279.
12. Li L., Hsu A., Moore PK. Actions and interactions of nitric oxide, carbon monoxide and hydrogen sulphide in the cardiovascular system and in inflammation - a tale of three gases! // Pharmacology, Therapeutics. 2009. Vol. 123. P 386-400.
13. Wu L., Wang R. Carbon Monoxide: endogenous production, physiological functions, and pharmacological applications // Pharmacol. Rev. 2005. Vol. 57, No. 4. P. 585-630.
14. Yang G., Wu L., Jiang B. [et al.] H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine -lyase // Science. 2008. Vol. 322. P. 587-590.
15. Zhang C.Y., Li X.H., Zhang T. [et al.] Hydrogen sulfide up-regulates heme oxygenase-1 expression in rats with volume overload-induced heart failure // Biomed. Rep. 2013. Vol. 1, No. 3. Р. 454-458.
Review
For citations:
Startseva M.S., Kotsuba A.E., Chertok V.M. The spatial organization of gas-transmitral neurons in the brain. Pacific Medical Journal. 2015;(2):39-43. (In Russ.)
Views:
217
Email this article 