Comparative optical characteristics of extracts from giant hyssop Tibetan and anise

Abstract

Methods. Absorption spectra of water and alcohol extracts from roots, leaves, flowers and seeds of anise and Tibetan giant hyssop were registered with a spectrophotometer UV-2501PC (Shimadzu), liquid chromatography of leaves infusions was carried out in a “acetonitrile -water” 9:1 with a chromatograph LC-10VP (Shimadzu) . Results. The absorption spectra of extracts from the roots of the anise and Tibetan giant hyssop, as well as flowers, seeds, leaves and water infusions match. For tincture 95 % ethanol leaves of anise giant hyssop spectrophotometric height maxima in the visible range of 2-2.3, 1.5-1.7, and chromatographic peaks and the value of specific absorption of 1.2 times greater than for Tibetan giant hyssop. Conclusions. Match of the majority of spectrophotometric characteristics of different extractants extracts from different parts of the Tibetan and anise giant hyssop indicates the presence of a part of these plants are similar in chemical nature of the substances. Tibetan and anise giant hyssop leaves significantly differ only on the content of chlorophyll. These spectrophotometric and chromatographic characteristics can be used in the development of normative documents for the manufacture of herbal remedies from giant hyssop.