Cooperation of hemopoietic stem cells and tumor cells in vitro
Abstract
Background. Proteomic differences hemopoietic stem cells from neural stem cell and tumor stem cells of glioblastoma imply that hemopoietic stem cells mainly hold over its regulatory property and could be used for treatment of glioma. Purpose of this research was the analyses of cell cooperation and influence of human hemopoietic stem cells on activity and proliferation of tumor cells of glioma in vitro. Methods. Was used mutual cultivation of glioma cells, fibroblast and hemopoietic stem cells stained with different fluorochroms. Cells was visualized with help of laser scan confocal microscopy. Calculation of number of fluoresce objects been carried out in automatically. Results. Was shown statistically proved slowdown of proliferation of neuroglioma cells cultivated with hemopoietic stem cells. In process of cultivation tumor cells amass fluorescers that was in cytoplasm of hemopoietic stem cells, furthermore the number of hemopoietic stem cells in culture decreased up to total vanishing to the end of experiment Conclusions. Hemopoietic stem cells cooperate actively and with glioma cells and exchanged with regulatory proteins, that slowdown neoplastic proliferation and move in time phase of logarithmic growth of tumors.
About the Authors
I. S. Bryhovetskiy
School of Biomedicine of Far East Federal University; Institute of Biology of sea of Zhirmundskogo A.V. FEB RAS; Clinic of Rehabilitation and Interventional Neurology and Therapy “Neurovita”
Russian Federation
Russian Federation
P. V. Mischenko
School of Biomedicine of Far East Federal University; Institute of Biology of sea of Zhirmundskogo A.V. FEB RAS
Russian Federation
Russian Federation
E. V. Tolok
School of Biomedicine of Far East Federal University; Institute of Biology of sea of Zhirmundskogo A.V. FEB RAS
Russian Federation
Russian Federation
R. Y. Hotimchenko
School of Biomedicine of Far East Federal University; Institute of Biology of sea of Zhirmundskogo A.V. FEB RAS
Russian Federation
Russian Federation
A. S. Bryhovetskiy
School of Biomedicine of Far East Federal University; Clinic of Rehabilitation and Interventional Neurology and Therapy “Neurovita”
Russian Federation
Russian Federation
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Review
For citations:
Bryhovetskiy I.S., Mischenko P.V., Tolok E.V., Hotimchenko R.Y., Bryhovetskiy A.S. Cooperation of hemopoietic stem cells and tumor cells in vitro. Pacific Medical Journal. 2014;(4):31-37. (In Russ.)
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