Evaluation of specific pharmacological activity of sapropel humin substances of Omsk region

Abstract

Background. Sapropel - natural raw materials that contain humin substances (HS) and has a proved biological activity. Research of general toxic properties of sapropel HS of Omsk region have shown their safety, and because of that was conducted the research of HS specific activity. Methods. Was explored sapropel, HS and activated humin substances (AHS). Antioxidant activity was tested on a model of adrenaline inhibiting autoxidation in alkaline medium in vitro in comparing with cevitamic acid. Study wound healing activity and anti-inflammatory activity conducted on experimental animals: models of burn wounds and limb edema. Antifungal properties was determined in vitro by the method of S.A. Vichkanova. Results. All materials demonstrated specific activity. HS ability to inhibit adrenaline free radical autoxidation was approximately 1.5, while AHS - 2 times higher than sapropels. Wound healing and anti-inflammatory activity of sapropel and HS were on the same level and did not exceed the activity of AHS. HS and AHS retards the growth of dermatomycosis and microsporia. Conclusions. Sapropel, HS and AHS have different degree of specific pharmacological activity, the most prosective for further study and practical application are activated humin substances.