EFFECT OF PERIPHERICALLY-INDUCED NEUROINFLAMMATION ON COGNITIVE FUNCTION IN YOUNG AND OLD MICE
Abstract
The article is devoted to experimental evaluation of the impact of peripherically-induced neuroinflammation on the severity of cognitive impairment, depending on age. Methods. Neuroinflammation has been induced in the 3- and 18-month-old white male mice with an intraperitoneal injection of bacterial lipopolysaccharides Escherichia coli 0111: B4 (Sigma Aldrich) 5 mg/kg. After 24 hours, the researchers determined locomotor activity and a working memory in Y-maze. Then the test animals were taken out of the experience and assessed the expression of the proteins GFAP and OX-42 on transverse hippocampal sections. Results. In 18-month-old mice there have been revealed more severe cognitive deficit and decreased locomotor activity compared with the group of “young” test animals. Immunohistochemical methods in hippocampus of 18-month-old mice showed more pronounced activation of microglia and astrogliosis. Conclusions. Aging is accompanied by increased tendency to neuroinflammation. Stimulation of the immune system by bacterial endotoxin increases the severity of inflammatory reactions in the brain of aged mice compared to young animals, regularly provoking neurodegeneration and cognitive deficit.
About the Authors
A. A. Tyrtyshnaya
Institute of Marine Biology named after A.V. Zhirmunskiy FEB RAS; Far Eastern Federal University
Russian Federation
Russian Federation
A. A. Zozulya
Institute of Marine Biology named after A.V. Zhirmunskiy FEB RAS
Russian Federation
Russian Federation
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Review
For citations:
Tyrtyshnaya A.A., Zozulya A.A. EFFECT OF PERIPHERICALLY-INDUCED NEUROINFLAMMATION ON COGNITIVE FUNCTION IN YOUNG AND OLD MICE. Pacific Medical Journal. 2014;(2):23-26. (In Russ.)
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