GALACTOSE AS A CONTRIBUTING FACTOR IN MULTIPLICATION OF PSEUDOTUBERCULOSIS BACTERIA IN VITRO AND IN VIVO

Summary - The authors study effects of galactose in reproduction of
enterobacteria and virulent properties of pseudotuberculosis bacteria
of galactose phenotype. Twenty strains of enterobacteria were
being cultivated under aerobic conditions using Luria broth (LB)
and LB with 0.5 % of galactose (LB+Gal). The reproduction dynamics
was estimated by studying optical density of suspensions. Noninbred
mice were infested with Yersinia pseudotuberculosis strain
known to carry recombinant plasmid with reporter system based
upon fluorescent protein. The authors have studied two groups by
10 animals undergone peroral introduction of bacterial suspension
cultivated in LB and LB+Gal broths. The biological material was
inoculated on nutrient agar with chloramphenicol to calculate the
number of colonies per 1 gram of fecals mass and liver in 48-72
hours. Most of Y. pseudotuberculosis strains were propagating more
intensively in the LB+Gal broth. The others, except Y. ruckeri and Y.
pestis Pestoides, showed the same characteristics. The strains of Escherichia
coli found in the LB+Gal broth demonstrated increasing
multiplication. The Salmonella and Shigella strain propagation did
not depend on carbohydrates. As observed, Y. pseudotuberculosis
bacteria cultivated in the LB+Gal broth propagated more intensively
in the digestive tract and demonstrated increasing capability
of penetrating epithelial barrier of bowels thus percolating the
internal environment.

Yersinia pseudotuberculosis, galactose, virulence, bacterial biomass

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