VARIABILITY OF FUNCTIONAL DOMAINS OF INVASION FACTORS AS MOLECULAR BASIS FOR POLYHOSTALITY OF SAPRONOSIS-INDUCED MICROORGANISMS

Summary - The sapronosis pathogens are characterised by polyhostality,
in other words, by a capability of existing and/or causing disease
in a wide range of hosts. The authors study variability of genes encoding
invasion factors using 86 Listeria monocytogenes isolates and 84 Yersinia pseudotuberculosis isolates. All genes have non-synonymous
mutations causing amino-acid replacements. The gene inv that encodes Y. pseudotuberculosis invasions has two non-synonymous mutations
that differ allele 1 from allele 2. Allele 1 dominates among Y. pseudotuberculosis isolates derived from sick people and the environment.
The isolates derived from gnawing animals have alleles 1
and 2 to be observed with the same frequency. The gene sequences inlA, inlB, ilnC and inlЕ encoding internaline proteins L. monocytogenes
have 6, 25, 8 and 48 non-synonymous mutations, respectively.
A range of replacements is characterised by all isolates belonging to
one serovar group. No replacements are observed in isolates of other
serovars. Besides, L. monocytogenes isolates are characterised by
uneven distribution of amino-acid replacements, depending on the
source of excretion. One of possible consequences of polymorphism
of invasion factors can be sampling of strains that more effectively
interact with certain variants of eukaryotic receptors and thus more
efficiently penetrate the cells of a mammal.

Listeria monocytogenes, Yersinia pseudotuberculosis, invasion factors, genetic variability

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