Zonulin, tumor necrosis factor alpha and nitric oxide in the diagnosis of IgE-dependent and IgE-independent food allergy in children
https://doi.org/10.17238/PmJ1609-1175.2017.2.18-21
Abstract
Objective. The purpose of this study was to determine the fecal level of zonulin, tumor necrosis factor alpha (TNFa) and nitric oxide metabolites in children with gastrointestinal forms of food allergy (FA) and to assess their pathogenetic significance in the diagnosis of IgE-dependent and IgE-independent FA. Methods. It was clinical and laboratory examines 39 children with food allergy to the cow milk protein at the age of 6 months to 3 years (Group I). The comparison group (Group II) consisted of 30 healthy children. The content of zonulin was determined using Immunodiagnostic (Germany) reagents using enzyme-linked immunosorbent assay (ELISA). Immunological study of fecal TNFa was performed with the help of BSM (USA) reagents using the enzyme-linked immunosorbent assay (ELISA) method. The concentration of NO metabolites in co-filters was determined using an enzyme immunoassay (ELISA) and the "Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit" (R&D Systems, USA). Results. Mean concentrations of zonulin in fecal samples in FA children and in control group children were 1.79±0.16 and 0.75±0.01 ng/ml, respectively (p<0.05). The level of TNFa (31,04±2,71 mmol/L) in FA children was in 7.6 times higher than in the control group. Analyze of the NO metabolites content in children with IgE-dependent and IgE-independent FA show the multidirectional changes in production of nitric oxide in children of different subgroups. Conclusions. Determination of fecal zonuline concentrations, TNFa and NO metabolites is an informative criterion for damage of the intestinal barrier in the development of food allergies in children. High numbers of these fecal markers are characteristic of the IgE-dependent form of the disease, increased zonulin, TNFa and decreased NO metabolites are characteristic of IgE-independent FA in children.
About the Authors
T. A. Shumatova
Pacific State Medical University
Russian Federation
Russian Federation
N. G. Prihodchenko
Pacific State Medical University
Russian Federation
Russian Federation
A. N. Ni
Pacific State Medical University
Russian Federation
Russian Federation
E. Y. Katenkova
Pacific State Medical University
Russian Federation
Russian Federation
L. A. Grigoryan
Pacific State Medical University
Russian Federation
Russian Federation
E. S. Zernova
Pacific State Medical University
Russian Federation
Russian Federation
S. N. Shishatskaya
Pacific State Medical University
Russian Federation
Russian Federation
References
1. Колесов С.А., Жукова Е.А., Коркоташвили Л.В. [и др.]. Метаболиты оксида азота, белок теплового шока 70 и провоспалительные цитокины у детей с заболеваниями кишечника // Международный журнал прикладных и фундаментальных исследований. 2014. № 4. С. 75-78.
2. Пампура А.Н. Пищевая аллергия у детей раннего возраста // Педиатрия. 2016. Т. 95, № 3. С. 152-157.
3. Шуматова Т.А., Приходченко Н.Г., Зернова Е.С. [и др.]. Полиморфизм генов фолатного цикла и эндогенные пептиды у детей с аллергией к белкам коровьего молока // Рос. вестник перинатологии и педиатрии. 2016. Т. 61, № 6. С. 113-118.
4. Шуматова Т.А., Шишацкая С.Н., Зернова Е.С. [и др.]. Современные маркеры в диагностике пищевой непереносимости у детей грудного возраста // Тихоокеанский медицинский журнал. 2015. № 3. С. 55-58.
5. Berin M.C., Sampson H.A. Mucosal immunology of food allergy // Curr. Biol. 2013. Vol. 6, No. 23. P. 389-400.
6. Good M., Sodhi C.P., Yamaguchi Y. [et al.]. The human milk oligosaccharide 2'-fucosyllactose attenuates the severity of experimental necrotising enterocolitis by enhancing mesenteric perfusion in the neonatal intestine // Br. J. Nutr. 2016. Vol. 116, No. 7. Р. 1175-1187.
7. Lee J., Bae E.H., Ma S.K., Kim S.W. Altered Nitric Oxide System in Cardiovascular and Renal Diseases // Chonnam. Med. J. 2016. Vol. 52, No. 2. Р. 81-90.
8. Mattila J.T., Thomas A.C. Nitric oxide synthase: non-canonical expression patterns // Front. Immunol. 2014. Vol. 9, No. 5. Р. 478.
9. Scurlock A.M., Vickery B.P., Hourihane J.O., Burks A.W Pediatric food allergy and mucosal tolerance // Mucosal Immunol. 2010. Vol. 3, No. 4. Р. 345-354.
10. Steele L., Mayer L., Berin M. C. Mucosal immunology of tolerance and allergy in the gastrointestinal tract // Immunol. Res. 2012. Vol. 54, No. 1. Р. 75-82.
11. Stepanova E.I., Skvarskaia E.A. Role of polymorphism NOsynthase gene in the pathogenesis of multifactorial diseases // Lik. Sprava. 2014. Vol. 5-6. Р. 47-55.
12. Velickovic K., Markelic M., Golic I. [et al.]. Long-term dietary L-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine // Eur. J. Nutr. 2014. Vol. 53, No. 3. Р. 813-821.
13. Yazji I., Sodhi C.P., Lee E.K. [et al.]. Endothelial TLR4 activation impairs intestinal microcirculatory perfusion in necrotizing enterocolitis via eNOS-NO-nitrite signaling // Proc. Natl. Acad. Sci. USA. 2013. Vol. 110, No. 23. Р. 9451-9456.
Review
For citations:
Shumatova T.A., Prihodchenko N.G., Ni A.N., Katenkova E.Y., Grigoryan L.A., Zernova E.S., Shishatskaya S.N. Zonulin, tumor necrosis factor alpha and nitric oxide in the diagnosis of IgE-dependent and IgE-independent food allergy in children. Pacific Medical Journal. 2017;(2):18-20. (In Russ.) https://doi.org/10.17238/PmJ1609-1175.2017.2.18-21
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