The technological concept of in vitro gamma-interferon tests to identify effector T cells that respond to stimulation with the Mycobacterium tuberculosis antigen and their use among children
https://doi.org/10.34215/1609-1175-2021-2-61-64
Abstract
Objective: To define the test place of T-Spot.TB gamma-interferon release in the algorithm of diagnosing and diagnostics of different onsets of tuberculosis infection among children and adloscents.
Methods: T-Spot.TB test results among children under 17 years old were examined as an alternative screening method (n=4096), in VI dispensary observation group (n=645) and among patients suffering tuberculosis (n=15).
Results: T-Spot.TB effectiveness as a screening test was 2.8 %. Matching this test’s results and the skin test with tuberculosis recombinant allergen (TRA) in a year of observation demonstrated negative result in 90.7 % of cases in the event of uncertain or weakly positive TRA reaction.
Conclusions: Child population tuberculosis screening under 7 years old should be held using Mantoux reaction, but from 8 to 17 years old – using TRA test. Gamma-interferon T-Spot. TB test can be held on a contract basis as an alternative child screening method if the legal representative refused to make recommended skin tests. Immunosuppression of various origins, allergies and obesity can be considered as medical indicators to prescribe this test for children and adolescents.
About the Authors
L. A. Bаryshnikovа
N.V. Postnikov Samara Regional Clinical Tuberculosis Dispensary
Russian Federation
Russian Federation
MD, PhD
154 Novo-Sadovaya St., Samara, 443068, Russian Federation
R. H. Fatykhova
Republican Clinical Tuberculosis Dispensary
Russian Federation
Russian Federation
MD, deputy chief physician
20 Shalyapina St., Kazan, 420049, Russian Federation
D. A. Kudlay
I.M. Sechenov First Moscow State Medical University;
NRC Institute of Immunology
Russian Federation
Russian Federation
MD, PhD, professor of the Pharmacology Department; leading researcher of the Laboratory of Personalized Medicine and Molecular Immunology No. 71
8/2 Trubetskaya St., Moscow, 119991, Russian Federation
24 Kahirskoe Shosse, Moscow, 115478, Russian Federation
A. P. Alekseev
Republican Clinical Tuberculosis Dispensary
Russian Federation
Russian Federation
MD, chief physician
20 Shalyapina St., Kazan, 420049, Russian Federation
M. N. Kabaeva
N.V. Postnikov Samara Regional Clinical Tuberculosis Dispensary
Russian Federation
Russian Federation
MD, chief physician
154 Novo-Sadovaya St., Samara, 443068, Russian Federation
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Review
For citations:
Bаryshnikovа L.A., Fatykhova R.H., Kudlay D.A., Alekseev A.P., Kabaeva M.N. The technological concept of in vitro gamma-interferon tests to identify effector T cells that respond to stimulation with the Mycobacterium tuberculosis antigen and their use among children. Pacific Medical Journal. 2021;(2):61-64. (In Russ.) https://doi.org/10.34215/1609-1175-2021-2-61-64
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