Virome of ancient wolf mummy (Canis lupus) as a potential source of virulent bacteriophages for phage therapy
A. E. Goncharov, 
Ya. A. Ahremenko,
D. V. Azarov,
M. J. Cheprasov,
S. E. Fedorov,
A. P. Solomenny,
V. V. Kolodzhieva
Ya. A. Ahremenko,
D. V. Azarov,
M. J. Cheprasov,
S. E. Fedorov,
A. P. Solomenny,
V. V. Kolodzhieva https://doi.org/10.34215/1609-1175-2023-1-35-37
Abstract
Aim. To investigate the potential for searching new virulent bacteriophages in the paleontological material extracted from the permafrost zone.
Material and methods. The virome structure of the colon content of the wolf pup mummy (Canis lupus) from the late Pleistocene was evaluated by means of shotgun metagenomic sequencing.
Results. The study demonstrated the predominance of Myoviridae tailed bacteriophages, including PhiKZ-like phages, in the structure of the virome.
Conclusion. The results of the study indicate the possibility of using paleontological material preserved in the ancient Arctic permafrost as a resource for searching and isolating new virulent bacteriophages.
About the Authors
A. E. Goncharov
Institute of Experimental Medicine; North-Western State Medical University named after I.I. Mechnikov; Saint-Petersburg State University
Russian Federation
Russian Federation
Saint-Petersburg
Ya. A. Ahremenko
M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Yana A. Akhremenko, Assoc. Prof., Head of the Course of Microbiology and Immunology, Department of Histology and Microbiology, Medical Institute
27 Oyunskogo str., Yakutsk, 677007
D. V. Azarov
Institute of Experimental Medicine; North-Western State Medical University named after I.I. Mechnikov
Russian Federation
Russian Federation
Saint-Petersburg
M. J. Cheprasov
M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Yakutsk
S. E. Fedorov
M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Yakutsk
A. P. Solomenny
Institute of Ecology and Genetics of Microorganisms of RAS Ural Branch
Russian Federation
Russian Federation
Perm
V. V. Kolodzhieva
North-Western State Medical University named after I.I. Mechnikov
Russian Federation
Russian Federation
Saint-Petersburg
References
1. Kerfahi D, Tripathi BM, Dong K, Kim M, Kim H, Slik JF, Adams JM. From the high Arctic to the equator: do soil metagenomes differ according to our expectations? Microbial ecology, 2019;77(1):168–85. doi: 10.1007/s00248-018-1215-z
2. Zablocki O, van Zyl L, Adriaenssens EM, Rubagotti E, Tuffin M, Cary SC, Cowan DA. High-level diversity of tailed Phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils. Appl Environ Microbiol 2014;80(22):6888–97. doi: 10.1128/AEM.01525-14
3. López-Bueno A, Tamames J, Velazquez D, Moya A, Quesada A, Alcami A High diversity of the viral community from an Antarctic Lake. Science 2009;326:858–61. doi: 10.1126/science.1179287
4. Grigoriev SE, Cheprasov MYu, Savvinov GN, Tikhonov AN, Novgorodov GP, Fedorov SE, Boeskorov GG, Protopopov AV, Plotnikov VV, Belolubsky IN, Protodiyakonov KE, Van der Plicht J. Paleontological and Archaeozoological Investigations in the Yana River Basin. Vestnik of the M.K. Ammosov North-Eastern Federal University. 2017;1(57):20–35 (in Russ.).
5. Danilova YA, Belousova VV, Moiseenko AV, Vishnyakov IE, Yakunina MV, Sokolova OS. Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage. Viruses. 2020;12:1197. doi: 10.3390/v12101197
6. Chaikeeratisak V, Nguyen K, Egan ME, Erb ML, Vavilina A, Pogliano J The phage nucleus and tubulin spindle are conserved among large pseudomonas phages. Cell Reports. 2017;20:1563–71. doi: 10.1016/j.celrep.2017.07.064
7. Malone LM, Warring SL, Jackson SA, Warnecke C, Gardner PP, Gumy LF, Fineran PC A jumbo phage that forms a nucleus-like structure evades CRISPR–Cas DNA targeting but is vulnerable to type III RNA-based immunity. Nat Microbiol. 2020;5:48–55. doi: 10.1038/s41564-019-0612-5
8. Legendre M, Bartoli J, Shmakova L, Jeudy S, Labadie K, Adrait A, Lescot M, Poirot O, Bertaux L, Bruley C, Couté Y, Rivkina E, Abergel C, Claverie JM. Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a Pandoravirus morphology. Proc. Natl. Acad. Sci. USA. 2014;111(11):4274–9. doi: 10.1073/pnas.1320670111.488
9. Legendre M, Lartigue A, Bertaux L, Jeudy S, Bartoli J, Lescot M, Alempic JM, Ramus C, Bruley C, Labadie K, Shmakova L, Rivkina, E, Couté Y, Abergel C. Claverie JM. In-depth study of Mollivirus sibericum, a new 30,000-y-old 490 giant virus infecting Acanthamoeba. Proc. Natl. Acad. Sci. USA. 2015;112(38):5327–35. doi: 10.1073/pnas.1510795112
Review
For citations:
Goncharov A.E., Ahremenko Ya.A., Azarov D.V., Cheprasov M.J., Fedorov S.E., Solomenny A.P., Kolodzhieva V.V. Virome of ancient wolf mummy (Canis lupus) as a potential source of virulent bacteriophages for phage therapy. Pacific Medical Journal. 2023;(1):35-37. (In Russ.) https://doi.org/10.34215/1609-1175-2023-1-35-37
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