Bioprofiles of Staphylococcus spp. isolated from prostate secretion in men with chronic bacterial prostatitis

Abstract

Objective. To characterize pathogenic pheno- and genoprofiles of staphylococci of different species, isolated from the secretion of the prostate gland in male patients with chronic bacterial prostatitis.

Materials and methods. The bacterial spectrum of microflora was studied by a bacteriological method; the species identification of microorganisms was carried out by mass spectrometry. Detection of genes determining pathogenicity factors was carried out by PCR. The biofilm-forming ability of staphylococci, as well as their anti-lysozyme, hemolytic, and adhesive activity, were determined by photometry.

Results. Staphylococci were found to be dominant in the pathogen structure of chronic bacterial prostatitis. These microorganisms, regardless of their species, had a pronounced pathogenic potential. Specific features in the bioprofiles of cultures of different species were determined according to the severity of the studied biological properties. Thus, the hemolytic activity and biofilm-forming ability was significantly higher in S. aureus. Conversely, CNS strains were characterized by high anti-lysozyme activity. A significant difference was noted in the prevalence of genes that determine pathogenicity factors in the studied staphylococci of different species.

Conclusion. The pathogenic bioprofile of staphylococci of various species isolated from patients with chronic bacterial prostatitis can be used as a criterion in the search and identification of the pathogen, as well as in the development of effective therapeutical approaches.