Decreased antioxidant capacity in children with diabetic ketoacidosis
https://doi.org/10.34215/1609-1175-2024-1-39-42
Abstract
Objective. To analyze the levels of antioxidant enzyme superoxide dismutase (SOD) and glutathione peroxidase (GP) against the background of diabetic ketoacidosis (DKA) in type 1 diabetes mellitus (DM) children and adolescents. Materials and methods. The study involved examination of 74 children: 50 DKA children (study group) and 24 relatively healthy children (control group). The study group children were divided into two subgroups: subgroup 1 included children with DKA against the background of type 1 DM onset (n=27), subgroup 2 consisted of children with DKA against the background of chronic type 1 DM (n=23). SOD and GP concentrations in blood plasma were determined in all children by enzyme immunoassay. The reliability between the data was estimated using the Mann-Whitney test, Kruskal-Wallis test and Spearman coefficient. Results. A significant decrease in SOD and GP in children with DKA was revealed as follows: 13130 [13005–18255] Pg/ml and 50.085 [42.02–70.325] Ng/ml, compared to controls: 16415 [13370–19935] Pg/ml and 84.695 [52.49–144.5] Ng/ml, respectively. Minimal SOD and GP were noted in patients with DKA at the background of chronic type 1 DM, compared to DM onset children. The study indicates a reliable correlation between age, duration of the disease, number of DKA in the history and low values of SOD and GP. Conclusion. Decreased antioxidant capacity was found in children with DKA in type 1 DM. SOD and GP can be considered in pediatric practice as markers of oxidative stress in DKA. In addition, an early detection of SOD and GP contributes to the efficient therapy of DKA in children and adolescents.
About the Authors
Yu. V. Bykov
Stavropol State Medical University ; City Children’s Clinical Hospital named G.K. Filippsky
Russian Federation
Russian Federation
Yuri V. Bykov, Cand. Sci. (Med.), assist. of Dept. of anesthesiology and intensive care with postgraduate course
310 Mira str., Stavropol, 355017 phone: + 7 (962) 443-04-92
V. A. Baturin
Stavropol State Medical University
Russian Federation
Russian Federation
Stavropol
A. P. Vorobyova
City Children’s Clinical Hospital named G.K. Filippsky
Russian Federation
Russian Federation
Stavropol
A. A. Vartanyan
Stavropol State Medical University
Russian Federation
Russian Federation
Stavropol
References
1. Janner M., Saner C. Impact of Type 1 Diabetes Mellitus on Bone Health in Children. Horm Res Paediatr. 2022;95(3):205–14. doi: 10.1159/000521627
2. Malyuzhinskaya NV, Shishimorov IN, Magnitskaya OV, Stepanenko KV, Polyakova OV, Klitochenko GV, Petrova IV, Emelyanova SA, Skiba AP. Pharmacotherapy possibilities of cardiovascular autonomic neuropathy in children with type 1 diabetes mellitus at the preclinical stage. Pharmacy and Pharmacology. 2022;10(1):93–103 (In Russ.). doi: 10.19163/2307-9266-2022-10-1-93-103
3. Bykov YuV. Diabetic ketoacidosis in children and adolescents: From pathophysiology to prevention. The Transbaikalian Medical Bulletin. 2021;2:85–95 (In Russ.) doi: 10.52485/19986173_2021_2_85
4. Otal Y, Kahraman FA, Haydar FG, Erel Ö. Dynamic thiol/ disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis. Eur J Clin Invest. 2018;48(10):e13006. doi: 10.1111/eci.13006
5. Kostopoulou E, Livada I, Partsalaki I, Lamari F, Skiadopoulos S, Rojas Gil AP, Spiliotis BE. The role of carbohydrate counting in glycemic control and oxidative stress in patients with type 1 diabetes mellitus (T1DM). Hormones (Athens). 2020;19(3):433–8. doi: 10.1007/s42000-020-00189-8
6. Hoffman WH, Siedlak SL, Wang Y, Castellani RJ, Smith MA. Oxidative damage is present in the fatal brain edema of diabetic ketoacidosis. Brain Res.2011;1369:194–202. doi: 10.1016/j.brainres.2010.10.085
7. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress. A concise review. Saudi Pharm J.2016; 24(5): 547–53. doi: 10.1016/j.jsps.2015.03.013
8. Suys B, de Beeck LO, Rooman R, Kransfeld S, Heuten S, Goovaerts I, Vrints C, de Wolf D, Matthys D, Manuel-y-Keenoy B. Impact of oxidative stress on the endothelial dysfunction of children and adolescents with type 1 diabetes mellitus: protection by superoxide dismutase? Pediatr Res. 2007;62(4):456–61. doi: 10.1203/PDR.0b013e318142581a
9. Ramakrishna V, Jailkhani R. Evaluation of oxidative stress in Insulin Dependent Diabetes Mellitus (IDDM) patients. DiagnPathol.2007;2:22. doi: 10.1186/1746-1596-2-22
10. Kueakhai P, Chaithirayanon K, Chaiwichien A, Samrit T, Osotprasit S, Suksomboon P, Jaikua W, Sobhon P, Changklungmoa N. Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis. Vet Parasitol. 2019;276:108979. doi: 10.1016/j.vetpar.2019.108979
11. El Amrousy D., El-Afify D., Shabana A. Relationship between bone turnover markers and oxidative stress in children with type 1 diabetes mellitus. Pediatr Res. 2021;89(4):878–81. doi: 10.1038/s41390-020-01197-5
12. Chaudhuri A., Umpierrez G.E. Oxidative stress and inflammation in hyperglycemic crises and resolution with insulin: implications for the acute and chronic complications of hyperglycemia. J Diabetes Complications. 2012; 26(4): 257–8. doi: 10.1016/j.jdiacomp.2012.04.016
13. Karavanaki K, Karanika E, Georga S, Bartzeliotou A, Tsouvalas M, Konstantopoulos I. Cytokine response to diabetic ketoacidosis (DKA) in children with type 1 diabetes (T1DM). Endocrine Journal.2011;58:1045–53. doi: 10.1507/endocrj.ej11-0024
Review
For citations:
Bykov Yu.V., Baturin V.A., Vorobyova A.P., Vartanyan A.A. Decreased antioxidant capacity in children with diabetic ketoacidosis. Pacific Medical Journal. 2024;(1):39-42. (In Russ.) https://doi.org/10.34215/1609-1175-2024-1-39-42
Views:
227
Email this article 