Effect of Yersinia pseudotuberculosis toxins on the reactivity of innate immunity cells

Abstract

Objective. The effect of thermolabile lethal toxin (TLT) and cytotoxic necrotizing factor (CNF) Yersinia pseudotuberculosis was studied on the enzymatic activity of peritoneal macrophages. Methods. The subjects of the study were TLT and CNF proteins isolated from Y. pseudotuberculosis 2517, III serovar strain (obtained from H. Mollaret, France). The primary culture of macrophages was obtained from peritoneal exudate of guinea pigs. We studied enzymes: 5'-nucleotidase, ATPase, lactate dehydrogenase, succinate dehydrogenase, cytochrome oxidase, nonspecific esterase, NO metabolites - nitrite. Results. Under the influence of TLT and CNF toxins, peritoneal macrophages were stimulated, as evidenced by low values of ectoenzymes. At the same time, under the influence of these toxins in phagocytes, stimulation of the oxygen-forming system enzymes was detected at the initial incubation times (1-6 h), and the stimulation of NO metabolites was at the end of the observation period. Against the background of increased activity of lysosomal enzymes (nonspecific esterase) in macrophages infected with TLT, the inhibition of lysosome functions was detected under the action of CNF toxin, which indicated suppression of the digestive capacity of phagocytes. Conclusions. CNF toxin of Y. pseudotuberculosis exerts a more pronounced cytotoxic effect on macrophages than TLT, as evidenced by activity induced of oxygen and nitroxide-forming systems, and low values of lysosomal enzymes.