Cytotoxicity evaluation of bioglasses doped with boron or bismuth and synthesized by different methods
https://doi.org/10.34215/1609-1175-2025-1-66-69
Abstract
Objective. To evaluate the cytotoxic properties of the obtained Bioglass 45S5 doped with 40 wt % Bi2O3 or 15 wt % B2O3.
Materials and methods. On cell culture of Ehrlich ascites adenocarcinoma, the metabolic activity of cells in a 96-well plate was evaluated by calorimetric MTT assay at threefold repetition.
Results. The influence of the production methods of a material on its toxic properties is confirmed. The pyrolysis of organic solutions is considered the most effective method for the synthesis of bioactive glasses. The concentration of samples of 12.5 mg/mL is considered promising for further studies.
Conclusion. The MTT assay shows an increase in the cytotoxicity parameter with the addition of boron ions to the composition of bioglass. At the same time, the introduction of bismuth demonstrates high viability parameters, which provides a basis for further development of biocompatible materials.
About the Authors
I. N. Chernenko
Pacific State Medical University
Russian Federation
Russian Federation
Olga V. Shevchenko, researcher at the Multidisciplinary laboratory center,
2 Ostryakova Ave., Vladivostok, Primorsky Territory, 690002
O. V. Shevchenko
Pacific State Medical University
Russian Federation
Russian Federation
2 Ostryakova Ave., Vladivostok, Primorsky Territory, 690002
D. N. Grishenko
Institute of Chemistry, FEB, RAS
Russian Federation
Russian Federation
Vladivostok
M. A. Medkov
Institute of Chemistry, FEB, RAS
Russian Federation
Russian Federation
Vladivostok
E. V. Markelova
Pacific State Medical University
Russian Federation
Russian Federation
2 Ostryakova Ave., Vladivostok, Primorsky Territory, 690002
References
1. Kostiv RYe, Matveeva NYu, Kalinichenko SG. Bioactive coatings on metallic alloys and stimulation of bone repair after fracture. Pacific Medical Journal. 2021;(2):31–36. (In Russ.). doi: 10.34215/1609-1175-2021-2-31-36
2. Khalid MD, Khurshid Z, Zafar MS, Farooq I, Khan RS, Najmi A Bioactive Glasses and their Applications in Dentistry. J. Pak. Dent. Assoc. 2017;26:32–38. doi: 10.25301/JPDA.261.32
3. Hench LL, Hench J., Greenspan DC Bioglass: A short history and bibliography. J. Aust. Ceram. Soc. 2004;40:1–42;2. doi: 10.1007/978-94-011-0541-5_1
4. Baino F, Hamzehlou S, Kargozar S Bioactive Glasses: Where Are We and Where Are We Going? J. Funct. Biomater. 2018;9:25. doi: 10.3390/jfb9010025
5. Nandi SK, Mahato A, Kundu B, Mukherjee P Doped Bioactive Glass Materials in Bone Regeneration. In: Zorzi A.R., de Miranda J.B., editors. Advanced Techniques in Bone Regeneration. IntechOpen; London, UK: 2016. doi: 10.5772/63266
6. Houaoui A, Lyyra I, Agniel R, Pauthe E, Massera J, Boissière M. Dissolution, bioactivity and osteogenic properties of composites based on polymer and silicate or borosilicate bioactive glass. Mater Sci Eng C. 2020;107:110340. doi: 10.1016/j.msec.2019.110340
7. Xia L, Ma W, Zhou Y, Gui Z, Yao A, Wang D, Takemura A, Uemura M, Lin K, Xu Y. Stimulatory effects of boron containing bioactive glass on osteogenesis and angiogenesis of polycaprolactone: in vitro study. Biomed Res Int. 2019;2019:8961409. doi: 10.1155/2019/8961409
8. Chen S, Michálek M, Galusková D, Michálková M, Švancárek P, Talimian A, Kaňková H, Kraxner J, Zheng K, Liverani L, Galusek D, R. Boccaccini A. Multi-targeted B and Co co-doped 45S5 bioactive glasses with angiogenic potential for bone regeneration. Mater Sci Eng C. 2020;112:110909. doi: 10.1016/j.msec.2020.110909
9. Divina R, Naseer KA, Marimuthu K, Alajerami YSM, AlBuriahi MS. Effect of different modifier oxides on the synthesis, structural, optical, and gamma/beta shielding properties of bismuth lead borate glasses doped with europium. J Mater Sci Mater Electron. 2020;31:21486–501. doi: 10.1007/s10854-020-04662-3
10. Tekin HO, Abouhaswa AS, Kilicoglu O, Issa SAM, Akkurt I, Rammah YS. Fabrication, physical characteristic, and gammaphoton attenuation parameters of newly developed molybdenum reinforced bismuth borate glasses. Phys Scr. 2020;95:115703. doi: 10.1088/1402-4896/abbf6e
11. Huang W, Day DE, Kittiratanapiboon K, Rahaman MN. Kinetics and mechanisms of the conversion of silicate (45S5), borate, and borosilicate glasses to hydroxyapatite in dilute phosphate solutions. J Mater Sci: Mater Med. 2006;17:583–96. doi: 10.1007/s10856-006-9220-z
12. Fabert M, Ojha N, Erasmus EP, Hannula M, Hokka M, Hyttinen J, Rocherullé J, Sigalasc I, Massera J. Crystallization and sintering of borosilicate bioactive glasses for application in tissue engineering. J Mater Chem B. 2017;5:4514–25. doi: 10.1039/c7tb00106a
13. Wang L, Long NJ, Li L, Lu Y, Li M, Cao J, Zhang Y, Xu S, Yang Z, Mao C, Peng M. Multi-functional bismuth-doped bioglasses: combining bioactivity and photothermal response for bone tumor treatment and tissue repair. Light Sci Appl. 2018;7:1. doi: 10.1038/s41377-018-0007-z
14. Prasad SS, Ratha I, Adarsh T, Anand A, Sinha PK, Diwan P, Annapurna K, Biswas K. In vitro bioactivity and antibacterial properties of bismuth oxide modified bioactive glasses. J Mater Res. 2018;33:178–90. doi: 10.1557/jmr.2017.442
Review
For citations:
Chernenko I.N., Shevchenko O.V., Grishenko D.N., Medkov M.A., Markelova E.V. Cytotoxicity evaluation of bioglasses doped with boron or bismuth and synthesized by different methods. Pacific Medical Journal. 2025;(1):66-69. (In Russ.) https://doi.org/10.34215/1609-1175-2025-1-66-69
Views:
58
Email this article 