Optimization of the Folin–Ciocalteu method for measuring phenolic antioxidants in the shoots of Crataegus pinnatifida Bunge
https://doi.org/10.34215/1609-1175-2025-4-79-85
Abstract
Objective. To optimize the Folin–Ciocalteu (FC) method for estimating phenolic antioxidants in the shoots of Crataegus pinnatifida Bunge using surface response methodology and the Box–Behnken design.
Materials and methods. The shoots of Crataegus pinnatifida Bunge were harvested in 2025 at the beginning of flowering from a natural population in Khabarovsk Krai. The measurements were performed using a UV-1700 spectrophotometer (Shimadzu, Japan). The response surface tool and the Box–Behnken design were applied to identify the combination of phenolic compound extraction factor levels that would yield the optimal response. Statistical processing of the results was carried out in accordance with the State Pharmacopoeia of the Russian Federation, 15th edition, OFS.1.1.0013 “Statistical processing of the results of physical, physicochemical, and chemical tests” using the Microsoft Office Excel 2010 and Statistica 6.0 software packages.
Results. For the shoots of Crataegus pinnatifida Bunge, the optimal parameters for the FC reaction have been determined as 0.5 ml of FC reagent and 5 ml of a 20% sodium carbonate solution, which ensure the accuracy, reproducibility, and stability of the reaction products. The response surface methodology and Box–Behnken design revealed that the highest yield of phenolic compounds occurs when the following extraction parameters are combined: extractant is 54.87% ethyl alcohol; raw material to extractant mass ratio is 1:783, and extraction time is 65.99 min.
Conclusion. Extraction conditions are optimized; a method for quantitative spectrophotometric determination of phenolic compounds in the shoots of Crataegus pinnatifida Bunge is developed, which is necessary for standardization of this type of raw material.
About the Authors
G. Ya. Mechikova
Far Eastern State Medical University
Russian Federation
Russian Federation
Galina Ya. Mechikova, Associate Prof. of Dep. of Pharmacy and Pharmacology
35 Muravyov-Amursky str., Khabarovsk, 680000, Russia
N. V. Matyushchenko
Far Eastern State Medical University
Russian Federation
Russian Federation
Khabarovsk
E. V. Slobodenyuk
Far Eastern State Medical University
Russian Federation
Russian Federation
Khabarovsk
References
1. Asfaw DE. Antioxidant properties of phenolic compounds to manage oxidative stress: A Review. J. Adv. Agron. Crop. Sci. 2023;103(2):1–16. doi: 10.17303/jacs.2023.2.103
2. Muscolo A, Mariateresa O, Giulio T, Mariateresa R. Oxidative Stress: the role of antioxidant phytochemicals in the prevention and treatment of diseases. Int. J. Mol. Sci. 2024;25(6):3264. doi: 10.3390/ijms25063264
3. Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV. An overview on the role of dietary phenolics for the treatment of cancers. Nutrition Journal. 2016;15(1):99. doi: 10.1186/s12937-016-0217-2
4. Carrillo JÁ, Zafrilla M.P., Marhuenda J. Cognitive function and consumption of fruit and vegetable polyphenols in a young population: is there a relationship? Foods. 2019;8(10):507. doi: 10.3390/foods8100507
5. Rahman MM, Rahaman MS, Islam MR, Rahman F, Mithi FM, Alqahtani T, Almikhlafi MA, Alghamdi SQ, Alruwaili AS, Hossain MS, Ahmed M, Das R, Emran TB, Uddin MS. Role of phenolic compounds in human disease: current knowledge and future prospects. Molecules. 2022;27(1):233. doi: 10.3390/molecules27010233
6. Deka H, Choudhury A, Dey BK. An overview on plant derived phenolic compounds and their role in treatment and management of diabetes. Journal of Pharmacopuncture, 2022;25(3):199–208. doi: 10.3831/KPI.2022.25.3.199
7. Khairnar SJ, Jadhav GB. Neuroprotective action of polyphenols and phenolic compounds: an overview. Biosci. Biotech. Res. Asia, 2023;20(3):793–816. doi: 10.13005/bbra/3132
8. Tatipamula VB, Kukavica B. Phenolic compounds as antidiabetic, anti-inflammatory, and anticancer agents and improvement of their bioavailability by liposomes. Cell Biochem Funct. 2021;39(8):926–44. doi: 10.1002/cbf.3667
9. Cherepanov SK. Vascular plants of Russia and neighbouring states (within the former USSR). Saint Petersburg: Mir i semya, 1995. 992p. (In Russ.).
10. Denisenko TA, Vishnikin AB, Tsiganok LP. Spectrophotometric determination of sum of phenolic compounds in plants using aluminum chloride, 18-molybdodiphosphate and FolinCiocalteu reagents. Analytics and Control. 2015;19(4):373–80 (In Russ.). doi: 10.15826/analitika.2015.19.4.012
11. Mechikova GYa, Stepanova TA, Zaguzova EV. Quantitative determination of total phenols in strawberry leaves. Khimiko-Farmatsevticheskii Zhurnal. 2007;41(2):38–41 (In Russ.). doi: 10.1007/s11094-007-0021-6
12. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 1965;16(3):144–58.
13. Palombini SV, Claus T, Maruyama SA, Carbonera F, Montanher PF, Visentainer JV, Gomes STM, Matsushita M. Optimization of a new methodology for determination of total phenolic content in rice employing Fast Blue BB and Quencher procedure. J. Braz. Chem. Soc. 2016;27(7): 1188–94. doi: 10.5935/0103-5053.20160013
14. Platzer M, Kiese S, Herfellner T, Schweiggert-Weisz U, Eisner P. How does the phenol structure influence the results of the FolinCiocalteu assay? Antioxidants. 2021;10(5):811. doi: 10.3390/antiox10050811
15. Martins GR, Monteiro AF, do Amaral FRL, da Silva AS. A validated Folin-Ciocalteu method for total phenolics quantification of condensed tannin-rich açaí (Euterpe oleracea Mart.) seeds extract. J. Food Sci. Technol. 2021;58(12):4693–702. doi: 10.1007/s13197-020-04959-5
16. Ferreira SLC., Bruns RE, Ferreira HS, Matos GD, David JM, Brandão GC, da Silva EGP, Portugal LA, dos Reis PS, Souza AS, dos Santos WNL. Box-Behnken design: An alternative for the optimization of analytical methods. Analytica Chimica Acta. 2007;597(2):179–86. doi: 10.1016/j.aca.2007.07.011
Review
For citations:
Mechikova G.Ya., Matyushchenko N.V., Slobodenyuk E.V. Optimization of the Folin–Ciocalteu method for measuring phenolic antioxidants in the shoots of Crataegus pinnatifida Bunge. Pacific Medical Journal. 2025;(4):79-85. (In Russ.) https://doi.org/10.34215/1609-1175-2025-4-79-85
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