Ability to biofilm formation in infectious agents isolated from patients of a large general children’s hospital
E. D. Savilov,
Y. A. Markova,
U. M. Nemchenko,
O. A. Noskova,
N. N. Chemezova,
E. A. Kungurtseva,
A. V. Dukhanina https://doi.org/10.34215/1609-1175-2020-1-32-35
Abstract
Objective: The study objective is to analyze biofilm formation in microorganisms isolated from patients of children’s hospital with severe purulent septic diseases.
Methods: The ability to biofilm formation was studied in microorganisms isolated from 32 patients aged from 1 to 15 y.o. with sepsis, acute hematogenous osteomyelitis, peritonitis, and pneumonia in intensive care unit of regional general children’s hospital. Blood, phlegm, bronchial and oropharyngeal washings, wound fluid, peritoneal fluid served as specimens.
Results: All tested strains have the ability to biofilm formation; moreover, the majority of them formed moderate biofilms.
Conclusion: The common spread of biofilm infections can be related to the most important factors of preservation and distribution of microorganisms in health facilities which significantly limit preventive and therapeutic measures.
About the Authors
E. D. Savilov
Scientific Centre of the Family Health and Human Reproduction Problems;
Irkutsk State Medical Academy of Continuing Education
Russian Federation
MD, PhD, main researcher of the Scientific Centre of the Family Health and Human Reproduction Problems (16 Timiryazeva St., Irkutsk, 664003
Russian Federation
MD, PhD, main researcher of the Scientific Centre of the Family Health and Human Reproduction Problems (16 Timiryazeva St., Irkutsk, 664003
Y. A. Markova
Siberian Institute of Physiology and Biochemistry of Plants
Russian Federation
Russian Federation
MD, PhD, head of the Laboratory of Plant-Microbial Interactions,
132 Lermontova St., Irkutsk, 664033
U. M. Nemchenko
Scientific Centre of the Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
MD, PhD, researcher,
16 Timiryazeva St., Irkutsk, 664003
O. A. Noskova
Scientific Centre of the Family Health and Human Reproduction Problems;
Irkutsk State Regional Children’s Hospital
Russian Federation
Russian Federation
junior researcher, 16 Timiryazeva St., Irkutsk, 664003
MD, deputy chief physician, 4 Gagarina St., Irkutsk, 664022
N. N. Chemezova
Scientific Centre of the Family Health and Human Reproduction Problems;
Irkutsk State Medical Academy of Continuing Education
Russian Federation
Russian Federation
researcher, 16 Timiryazeva St., Irkutsk, 664003
MD, PhD, associate professor, Epidemiology and Microbiology Department, 3 Karl Marks St., Irkutsk, 664003
E. A. Kungurtseva
Scientific Centre of the Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
PhD, junior recearcher,
16 Timiryazeva St., Irkutsk, 664003
A. V. Dukhanina
Scientific Centre of the Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
PhD, engineer,
16 Timiryazeva St., Irkutsk, 664003
References
1. Kovalishena OV, Blagonravova AS, Vorobyeva ON, Ermolyeva SA, Poslova LYu, Shprykova ON. Topical issues of epidemiological surveillance behind hospital infections. Remedium Privolzhye. 2008;1:49–51 (In Russ).
2. The national concept of prevention of the infections connected with delivery of health care, 2011. (In Russ). URL: http://www.garant.ru/products/ipo/prime/doc/70000121/ (Accessed Apr 24, 2019).
3. Anganova EV, Savilov ED, Ushkareva OA, Ablov AM, Duhanina AV. Ability of pathogenic and opportunistic enterobakteriya to formation of biofilms. Acta Biomedica Scientifica. 2014;5:34–7 (In Russ).
4. Yarets Yu, Shauchenka N. A new method for the bacterial biofilms analysis in medicine. Science and Innovations. 2016;10: 64–68 (In Russ).
5. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol. 1985;22(6):996–1006.
6. Gostev VV, Sidorenko SV. Bacterial biofilms and infections. Magazine of an Infectology. 2010;3(2):4–15 (In Russ).
7. Kirov SM, Webb JS, O’May CY, Reid DW, Woo JK, Rice SA, Kjelleberg S. Biofilm differentiation and dispersal in mucoid Pseudomonas aeruginosa isolates from patients with cystic fibrosis. Microbiology. 2007; 153:3264–74.
8. Eze EC, Chenia HY, El Zowalaty ME. Acinetobacter baumannii biofilms: Effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments. Infection and Drug Resistance. 2018; 11:2277–99.
9. Gupta TT, Karki SB, Matson JS, Gehling DJ, Ayan H. Sterilization of biofilm on a titanium surface using a combination of nonthermal plasma and chlorhexidine digluconate. BioMed Research International. 2017; doi: 10.1155/2017/6085741
10. Yu W, Hallinen KM, Wood KB. Interplay between antibiotic efficacy and drug-induced lysis underlies enhanced biofilm formation at subinhibitory drug concentrations. Antimicrobial Agents and Chemotherapy. 2018;62(1):16–7.
11. Johani K, Abualsaud D, Costa DM, Hu H, Whiteeley G, Deva A, Vickery K. Characterization of microbial community composition, antimicrobial resistance and biofilm on intensive care surfaces. J Infect Public Health. 2018;11(3):418–24.
12. Kamaruzzaman N, Tan LP, Mat Yazid KA, Saeed SI, Hamdan RH, Choong SS, et al.. Targeting the bacterial protective armour; Challenges and novel strategies in the treatment of microbial biofilm. Materials (Basel). 2018;11(9); doi: 10.3390ma11091705
13. Mohamed SH, Salem D, Azmy M, Fam NS. Antibacterial and antibiofilm activity of cinnamaldehyde against carbapenem-resistant Acinetobacter baumannii in Egypt: In vitro study. J App Pharm Sci. 2018;8(11):151–6.
Review
For citations:
Savilov E.D., Markova Y.A., Nemchenko U.M., Noskova O.A., Chemezova N.N., Kungurtseva E.A., Dukhanina A.V. Ability to biofilm formation in infectious agents isolated from patients of a large general children’s hospital. Pacific Medical Journal. 2020;(1):32-35. (In Russ.) https://doi.org/10.34215/1609-1175-2020-1-32-35
Views:
504
Email this article 