Use of the method of computer images combining for topochemical carting of the brain neurons

Abstract

Background. Absence of accessible and objective methods for studying of the dimensional neuron organization of various mediator usage leads to inconsistent estimations of the type of the relations between these cells in unique or multiple nucleus in any brain formation. Methods. On consecutive cuts of the longitudinal brain the neural structure of reticular lateral nucleus is studied by methilene blue coloring and also by the methods for revealing of norepinephrinergic and nitroxodergic neurons. The received nucleus images displayed on the monitor screen then were combined and on the basis of the received data the topochemical neuron carting was done. Results. At combination of the nucleus images received on the consecutive cuts after their coloring by the proper methods not only the features of the dimensional neuronal organization were revealed but also rather distinctively the differences of the form, the sizes and numbers of each cell population. Conclusions. The suggested method is accessible and simple in use, allows obtaining quickly the objective data for exact and detailed topochemical carting of the various mediator systems in one or several structural brain formations.