THE WAY OF A QUICK IDENTIFICATION OF BACTERIA GENUS LISTERIA AND PATHOGENIC SPECIES OF LISTERIA MONOCYTOGENES BY MEANS OF THE MULTIPLEX POLYMERASE CHAIN REACTION
Abstract
The wide polymorphism of the Listeria microbes and impermanence of their biochemical and biological characteristics inherent to the freshly isolated cultures of Listeria requires the development of quite new approaches to their typing. Methods. Investigated possibility of the use of the multiplex polymerase chain reaction (PCR) with primers limiting the sequences of genes of Phosphoribosyl pyrophosphate synthetase (prs) and Phosphatidylinositol-specific phospholipase (plcA) for a quick identification of bacteria genus Listeria along with a quick differentiation of the pathogenic species of Listeria monocytogenes. Assessment of efficacy of the PCR tested on 117 Listeria cultures isolated from the different foodstaff and organs of the murine rodents. Results. The use of the multiplex PCR enabled to relate 38 of Listeria cultures to genus L. monocytogenes while the rest to genus Listeria spp. that coincided with the results of microbiological typing and testing by means of LISTER System. Conclusions. The proposed multiplex PCR system enables to relatively quickly (within 3 hours) to screen for the Listeria-suspected colonies and to simultaneously identify L. monocytogenes in the samples under research and can be used for nmonitoring Listeria infection during microbiological and biological research.
About the Authors
S. M. Starodumova
G.P. Somov Institute of Epidemiology and Microbiology of the SB of the Russian Academy of Medical Sciences
Russian Federation
Russian Federation
E. A. Zaitseva
G.P. Somov Institute of Epidemiology and Microbiology of the SB of the Russian Academy of Medical Sciences; Pacific State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Starodumova S.M., Zaitseva E.A. THE WAY OF A QUICK IDENTIFICATION OF BACTERIA GENUS LISTERIA AND PATHOGENIC SPECIES OF LISTERIA MONOCYTOGENES BY MEANS OF THE MULTIPLEX POLYMERASE CHAIN REACTION. Pacific Medical Journal. 2014;(1):95-97. (In Russ.)
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