THE INHIBITING EFFECT OF PHOTODYNAMIC THERAPY AND NOVEL RECOMBINANT HUMAN ENDOSTATIN ON THE N VIVO GROWTH OF U251 HUMAN GLIOMA XENOGRAFTS
Abstract
Endostar, a novel recombinant human endostatin expressed in Escherichia coli, was approved by the State FDA in China. To investigate the effect of endostar and photodynamic therapy (PDT) on the in vivo growth of U251 glioma. Seven days after inoculation with U251 cells, nude mice with MRI-confirmed glioma were randomly assigned to 4 groups: PDT+endostar group; PDT group; endostar group and control group. In the PDT group, survival prolonged, accompanied by an increase in apoptosis, when compared with the control group. Furthermore, these changes were more pronounced in the PDT+endostar group. After PDT, hypoxia inducible factor-1a (HIF-1a) and vascular endothelial growth factor A (VEGF-A) expression was markedly increased and after endostar treatment, HIF-1a and VEGF-A expression was significantly reduced. PDT, in combination with endostar, can significantly inhibit the growth of U251 glioma. This approach may represent a promising strategy in the treatment of malignant tumors.
About the Authors
S. Hu
Second Affiliated Hospital
Russian Federation
Russian Federation
Qi. Zhan
Fourth Affiliated Hospital of Harbin Medical University
Russian Federation
Russian Federation
Wu. Yue
Fourth Affiliated Hospital of Harbin Medical University
Russian Federation
Russian Federation
References
1. Barnett F.H., Scharer-Schuksz M., Wood M. et al. Intra-arterial delivery of endostatin gene to brain tumors prolongs survival and alters tumor vessel ultrastructure // Gene Ther. 2004. Vol. 11. P. 1283-1289.
2. Bhuvaneswari R., Yuen G.Y., Chee S.K., Olivo M. Hypericin-mediated photodynamic therapy in combination with Avastin (bevacizumab) improves tumor response by downregulating angiogenic proteins // Photochem. Photobiol. Sci. 2007. Vol. 6. P. 1275-1283.
3. Candolfi M., Curtin J.F., Nichols W.S. et al. Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression // J. Neurooncol. 2007. Vol. 85. P. 133-148.
4. Chen Q., Huang Z., Chen H. et al. Improvement of tumor response by manipulation of tumor oxygenation during photodynamic therapy // Photochem. Photobiol. 2002. Vol. 76. P. 197-203.
5. Ferrario A., von Tiehl K.F., Rucker N. et al. Antiangiogenic treatment enhances photodynamic therapy responsiveness in a mouse mammary carcinoma // Cacer Res. 2000. Vol. 60. P. 4066-4069.
6. Folkman J. Anti-angiogenesis: new concept for therapy of solid tumors // Ann. Surg. 1972. Vol. 175. P. 409-416.
7. Forsythe J.A., Jiang B.H., Iyer N.V. et al. Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1 // Mol. Cell. Biol. 1996. Vol. 16. P. 4604-4613.
8. Hu S.S., Cheng H.B., Zheng Y.R. et al. Effects of photodynamic therapy on the ultrastructure of glioma cells // Biomed. Environ. Sci. 2007. Vol. 20. P. 269-273.
9. Huita W.U., Jie Deng, Shiying Y.U. et al. The inhibitory effects of Rh-endostatin (YH-16) in combination with radiotherapy on lung adenocarcinoma A549 in mice and the underlying mechanisms // J. Huazhong. Univ. Sci. Technol. 2010. Vol. 30. P. 108-112.
10. Jin F., Gao C., Zhao L. et al. Using CD133 positive U251 glioblastoma stem cells to establish nude mice model of transplanted tumor // Brain Res. 2011. Vol. 1368. P. 82-90.
11. Madsen S.J., Kharkhuu K. Hirschberg H. Utility of the F98 rat glioma model for photodynamic therapy // J. Environ. Pathol. Toxicol. Oncol. 2007. Vol. 26. P. 149-155.
12. O’Reilly M.S., Boehm T., Shing Y. et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth // Cell. 1997. Vol. 88. P. 277-285.
13. Schmidt N.O., Ziu M., Carrabba G. et al. Antiangiogenic therapy by local intracerebral microinfusion improves treatment efficiency and survival in an orthotopic human glioblastoma model // Clin. Cancer Res. 2004. Vol. 10. P. 1255-1262.
14. Smith J.S., Chang E.F., Lamborn K.R. et al. Role of extent of resection in the long-term outcome of low-grade hemispheric gliomas // J. Clin. Onco. 2008. Vol. l26. P. 1338-1345.
15. Zhou Q., Olivo M., Lye K.Y. et al. Enhancing the therapeutic responsiveness of photodynamic therapy with the antiangiogenic agents SU5416 and SU6668 in murine nasopharyngeal carcinoma models // Cancer Chemother. Pharmacol. 2005. Vol. 56. P. 569-577.
Review
For citations:
Hu S., Zhan Q., Yue W. THE INHIBITING EFFECT OF PHOTODYNAMIC THERAPY AND NOVEL RECOMBINANT HUMAN ENDOSTATIN ON THE N VIVO GROWTH OF U251 HUMAN GLIOMA XENOGRAFTS. Pacific Medical Journal. 2013;(4):67-71. (In Russ.)
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