Respiratory burst and metabolism of blood neutrophils in patients with different tolerance to acetylsalicylic acid with an acute coronary syndrome
https://doi.org/10.17238/PmJ1609-1175.2016.4.61-65
Abstract
The research objective is an analysis of the characteristics of a respiratory burst and the activity of NAD(P)-dependent dehydrogenases in blood neutrophils in sensitive and resistant to acetylsalicylic acid (ASA) patients with acute coronary syndrome (ACS). Methods. The study included 53 patients with ACS. The resistance / sensitivity to ASA was studied in vitro, incubating the platelets to adenosine diphosphate and ASA. Status respiratory burst of neutrophils was studied by chemical luminescence, and the activity of NAD(P)-dependent dehydrogenases - by bioluminescent method. Results. In patients resistant to ASA the rate of synthesis of primary and secondary reactive oxygen lowered, the index of luminol- dependent activation of neutrophils decreased, of substrate stimulation of glycolysis and glucose oxidation by the pentose phosphate pathway increased. Conclusions. In patients with ACS resistant to ASA is marked a decrease in functional activity and disturbances in the metabolism of neutrophils. The possible cause of the condition described above is energy losses of a cell, associated with the increase of the intensity and substrate stimulation of glycolysis and glucose oxidation by the pentose phosphate pathway.
About the Authors
I. Yu. Grinstein
Krasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetsky
Russian Federation
Russian Federation
A. A. Savchenko
Research Institute of Medical Problems of the North
Russian Federation
Russian Federation
Yu. I. Grinstein
Krasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetsky
Russian Federation
Russian Federation
I. I. Gvozdev
Research Institute of Medical Problems of the North
Russian Federation
Russian Federation
M. M. Petrova
Krasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetsky
Russian Federation
Russian Federation
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Review
For citations:
Grinstein I.Yu., Savchenko A.A., Grinstein Yu.I., Gvozdev I.I., Petrova M.M. Respiratory burst and metabolism of blood neutrophils in patients with different tolerance to acetylsalicylic acid with an acute coronary syndrome. Pacific Medical Journal. 2016;61(4):61-65. (In Russ.) https://doi.org/10.17238/PmJ1609-1175.2016.4.61-65
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