Pathogenicity factors and antibiotic resistance strains of Enterococcus faecalis isolated from prostate secretions in males with chronic bacterial prostatitis

Aim. To characterize pathogenic phenophiles and genoprophiles, as well as antibiotic c resistance of Enterococcus faecalis strains isolated from prostate secretions in males with chronic bacterial prostatitis (CBP).

Materials and methods. Enterococci were isolated using standard bacteriological methods on the Schaedler broth and identified by mass spectrometry. The genetic determinants encoding pathogenicity and antibiotic resistance factors were determined by PCR. The ability of enterococci to inactivate lysozyme (ALA), form biofilms, exhibit hemolysis and adhesion was determined by the photometric method; the penetrance and expressiveness of sIgA protease and anti-cytokine activities were evaluated by enzyme immunoassay.

Results. The isolated strains E. faecalis had a pronounced pathogenic potential, being characterized by the presence of virulence factors (hemolytic activity, adhesive ability) and persistence (ALA, BPO), the ability to inactivate proinflammatory cytokines and sIgA, variable antibiotic resistance with resistance to amikacin and kanamycin. The determinants responsible for the synthesis of proteolytic enzymes (gelE, sprE) and cytolysin (a complex of cyl operon genes), as well as those responsible for evading macroorganism immunity effectors (esp) and the synthesis of adhesion proteins (asa), were identified in the genotype of E. faecalis cultures. The presence of genetic determinants of antibiotic resistance aph(3’)-IIIa, tetM, vanA was found in all isolates.

Conclusion. The obtained results on pathogenic bioprophiles of clinical enterococcal strains can be used to improve approaches to CBP diagnosis. The data on antibiotic resistance (at the level of phenotype and genotype) should be taken into account in the empirical and personalized choice of drugs for a combined treatment of CBP patients.

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