As a result of long-term supervision in the centers of the Hantavirus infection with use of modern methods of research the prominent aspects of the natural reservoir of the hemorrhagic fever with nephritic syndrome are proved. The characteristic of structural components in the centers of different landscape zones and the data on genetic structure Hantavirus and rodents — carriers of viruses is submitted. The factors influencing a condition and activity of functioning of the natural center are designated. Conditions and the periods of the most active duplication Hantavirus infection at rodents and its allocation in an environment are determined.

As a result of complex clinical, epidemiological, immune and molecular-genetic researches circulation in area Sochi of new subspecies of virus Dobrava DOBV-Ap which basic owner and a source of infection of people is Caucasian wood mouse Apodemus ponticus has been found. The diseases caused by these virus at people had the certain differences of clinical signs were more severe than of the hemorrhagic fever with nephritic syndrome at inhabitants of the central areas of Russia, caused by subspecies DOBV-Aa.

Hantavirus infection is known to two forms - hemorrhagic fever with nephritic syndrome and Hantavirus cardiopulmonary syndrome. The given literatures and own researches for the benefit of viral-immune concepts of the pathogenesis of the Hantavirus infection are resulted. The development of reactions of an immune inflammation causes hemodinamic changes in target-organs. Equation of T-cellular effects at easy and moderate forms of disease is favorable for healing. Deep structural dysbalance in cytokines and a cellular immunity results in formation of multiorgan insufficiency, severe clinical signs and an opportunity of an unsuccessful outcome.

The characteristic of the natural reservoir of Hantavirus infection connected to viruses of four genotypes (Topographers, Puumala, Dobrava/Belgrad and Tula), circulating in ecologic systems of various landscape zones in three areas of Western Siberia is given. Distribution of the natural reservoir of all four groups of virus has the expressed mosaic character. Arctic Hantavirus of genotype of Topographers is associated with Siberian rodents in geographically removed territories. In Western Siberia viruses Puumala are submitted by a variant connected to populations of red mice. The natural reservoir of the hemorrhagic fever with nephritic syndrome, associated with Hantavirus infection Dobrava/Belgrad, are related for a wood zone of region, connected to populations of the field mouse and territorially blocked with the reservoir of Hantavirus Puumala. The genotype Tula is submitted to two variants connected specific rodents. The activity of the epizootic process in the natural centers of Hantavirus is caused by features of ecology of rodents.

Puumala Hantavirus infection forms ten clusters, having relative geographical specificity. Stellate structure of trees assumes an old divergence of the given groups. In each group (except for Siberian) connection with one kind of rodents of owners is kept. Comparing intra- and intercluster genetic distances and levels of homology between sequences, it is possible to assume an opportunity of absolutely recent «switching» of viruses Puumala with Clethrionomys glareolus on Clethrionomys rufocanus in Western Siberia where the genetic affinity of these rodents can be caused by their long coexistence in overlapped ecological niches.

The modern data on participation of monocytas in pathogenesis of hantavirus infection are resulted. Proofs are submitted, that the susceptibility of monocytes to Hantavirus grows at last stage of a differentiation. By virtue of phylogenetic uniformity of these cells, the data received at research of their interaction with hantavirus, it is possible to extrapolate on monocytes of the mammal class. It is specified, that hantavirus resistant to influence of the monocytes and capable to an endocellular reproduction in them, breaking thus the biological barrier interfering distribution of the activator from the primary center of an infection and protecting sensitive cells of various organs.

DNA-vaccination methods are based on the introduction of plasmid DNA encoding the pathogen's protein to organism. This approach allows obtaining the complete immune response to different bacterial and viral antigens. At the review, perspective is considering of DNA-vaccine creation on the base of HFRS-exciting structural genes, and problems following such a vaccines creation are emphasized.

In article the data about coincidence of the natural reservoirs of tularemia, leptospirosis and Hantavirus infection and the characteristic of their feachers are submitted. On landscape to geographical characteristics they belong to marsh and field types. Circulation of activators of the above-stated infections is provided basically on rodents of two kinds - the field mouse and Far East mouse. Presence of all of three forms can be found in marsh type of the reservoir more often and less often — in field.

The analysis of case records of 130 patients by hemorrhagic fever with nephritic syndrome, taking place in infectious hospitals of Primorsky Krai is done. At the analysis of a degree of incidence of an infection in 2005—2007 there were no easy forms of disease that, probably, is connected to small vigilance of doctors of a primary link. Clinical current of disease has classical signs at the hemorrhagic fever with nephritic syndrome, caused by serotypes Hantaan and Amur. For hemorrhagic fever with nephritic syndrome, associated with virus Seoul, cyclicity is less peculiar and polymorphism of clinical signs, including symptoms of lung and liver insufficiency is more often.

By the method of a return transcription - polymerase chain reaction with the subsequent 8 strains of Hantavirus, al- located from mice sorts Microtus from Far East region of Russia have been investigated. Independent zoological definition of a kind of rodents of carriers and the analysis of mitochondrial DNA have been done. The comparative analysis established nucleotide sequences of fragments of M of a segment virus of genome of the Hantavirus and m-DNA of mice with nucleotide sequences submitted in database GenBank, has revealed circulation of two genotypes KBRV and VLAV in two kinds of rodents — carriers of viruses. Our researches for the first time have revealed circulation of genotypes KBRV in mice M. maximowiczii and VLAV in mice M. fortis in territory of the Amur area, and also have confirmed circulation VLAV in mice M. fortis in territory of Primorsky Krai.

A total of 6462 small mammals of 10 species were collected from the Primorye region in 2001—2007 and examined by ELISA and IFA to detect hantavirus antigen and specific antibodies. Hantavirus circulation was revealed in 7 species. Index of epizootological potential (Iep) was offered for to evaluation of species of rodents as the potential source of hantavirus in periods of different activity of epizootic process in populations of rodents-reservoirs of etiologic agents of hemorrhagic fever with renal syndrome. Annual epidemic situation in Primorye is determined by epizootological potential of rodent's species of genus Apodemus — ecological hosts of pathogenic hantaviruses Far East and Amur. The results of correlation analysis showed the effectiveness index Iep in carrying out of zoological and epidemic monitoring in natural foci of hantaviral infection.

The structures of the population changes in rodents — carriers of pathogenic genotypes/serotypes of hantavirus and their number in successions of the basic vegetative formations caused by anthropogenous influence (fires) are submitted. It is shown, that changes of the population of rodents in degradation successions quite often result in growth of potential danger of Hantavirus infection. The suggested circuits of the connected transformation of vegetation and the population of rodents can form a basis for the forecast epizootic and an epidemiological condition of investigated territory.

Long-term dynamics of activity of epizootic process and intensity of intraspecific aggression was studied in Apodemus рeninsulaе and Apodemus agrarius rodent populations — reservoirs of etiologic agents of hemorrhagic fever with renal syndrome (HFRS) in Primorye region. It has been shown that the direct transmission mode of hantaviruses, associated with aggressive contacts in populations of different Apodemus rodents, is more significant in spring-summer season in the time of temporal high population number, largely among sexual maturity males. Observation of the level of intraspecific aggression in populations of hantavirus rodent-host may be recommended as one of the additional tests to assess the activity of HFRS natural foci.

The viral activity of the strains of hantavirus infection of two gene variants — Far East virus Hantaan and Vladivostok virus Seoul, allocated from wild mice rodents and sick people from the centers of two epidemiological types of Primorsky Krai is investigated. It is shown, that gene variant strains Far East, despite of their origin, possess the high viral activity for newborn white mice, but differ on viral activity for newborn white rats. Gene variant Vladivostok, allocated from sick people, aviral for the investigated animals. Regional strains differed on the biological properties from high viral strains of 76 118 virus Hantaan and SR1 1 virus Seoul that can be connected to distinctions at their genome level.

Distribution of bacteria Listeria among mice rodents caught in territory of Primorsky Krai is investigated, and separate factors of pathogenicity of the activator, necessary infections at the certain stages and resulting in persisting it in an organism of rodents are revealed. It is marked, that among the surveyed rodents Listeria have been found out in the field mouse, it is East-Asian mice, red mice. Over the group of animals on a parameter of Listeria infection was dominated with the field mouse (Apodemus agrarius), living on flat territory of Primorsky Krai and among it — L. monocytogenes which is pathogenic for the human and animals was allocated. Researches have shown existence of epizootic process of Listeria in a population of mice rodents in territory of Primorsky Krai which under certain conditions can be a source of an infection.

At 20 patients with the hemorrhagic fever with nephritic syndrome the step technique of virazol therapy (intravenous introduction of 16 mg/kg 4 times day) within 3 days with the subsequent veroribavirin per os of (1000 mg day — 5 days) is applied. The beginning of therapy at all patients was in 4th-5th day of illness. At the patients receiving virazol, the body temperature was faster normalized, stomach pain disappeared, there was less oliguria duration, hemorrhagic syndrome was absent. Duration of RNA detection of hantavirus infection in blood of 20 patients receiving virazol and ribavirin, was less, than at patients not receiving antiviral medications. Positive influence of virazol on immuno-pathogenesis of the infections that is confirmed with the data of a cytokine level is found.

On the basis of the analysis of lethal cases the hemorrhagic fever with nephritic syndrome, observed in Primorsky Krai and the Amur area, are determined features of pathological process at this infection in Far East region. It is shown, that basic pathogenetic path of the hemorrhagic fever with nephritic syndrome is system defeat of micro vessels, caused by expressed toxic properties of hantavirus during the period of virusemia. Thus hantavirus infection renders direct cytolytic action on endothelial cells. It is marked, that the destructive necrotic changes in target organs (especially in kidneys and lungs) carry not only secondary character owing to expressed hemodynamic frustration, but also are connected to direct action of a virus. The specific features of patho-morphology of the hemorrhagic fever with nephritic syndrome is the minimal expressiveness of cellular reaction peculiar to a typical inflammation in tissue that is caused by misbalance of inflammatory cytokines developing at this infection.

The analysis of clinical features and outcomes at severe hemorrhagic fever with nephritic syndrome is submitted. Some pathogenetic features of illnesses and as the common features in kidneys, hearts and lung lesions are revealed. Macro- and microscopic changes of the basic target-organs including on the posthumous X-rays are analyzed. It is shown, that multi-organ insufficiency at hemorrhagic fever with nephritic syndrome is determined not only by micro-vessels lesions, but also by large intraorgan vessels.

Results of studying of changes of the anti-hemo-agglutinating and neutralization antibodies at patients with the hemorrhagic fever with nephritic syndrome are submitted. By results of reaction of braking of hemagglutination on occasion at patients with moderate infection the more expressed immune answer is revealed, especial in an initial stage of illness, as against severe forms. The importance of use of reaction of braking of hemagglutination for the information on participation of the certain serotypes/genotypes of hantavirus in epidemic process is shown. Changes of specific antibodies at patients with various forms of disease in reaction of neutralization is investigated. The level of antibodies titer essentially did not depend on severity; the tendency to its higher value at patients with easy forms of disease however was traced in peak of illness and a phase of recovery.

The retrospective epidemiological analysis of 530 case records of patients with the hemorrhagic fever with nephritic syndrome for 1986-2006 from the reservoirs of circulation of viruses Hantaan and Amur is done. The received data show, that severity of an infection depends on duration and conditions of contact with pathogen. The work in conditions of high dusty or long stay in a wood result in more severe hantavirus infection. The estimation of the occupation of patients and conditions of infection has allowed allocating the group of the increased risk of Hantavirus infection in Primorsky Krai.

The virus inhibiting activity of the fukoidans of the brown seaweed in view of their structural features in experiences in vivo at hantavirus infection at mice is investigated. It is found, that fukoidans from brown seaweed Laminaria japonica and Laminaria cichorioides possess property to suppress the hantavirus infection to the peritoneal macrophages of mice mainly in an initial phase of infectious process. The positive effect of fukoidans from fukus and laminaria seaweed has been to some extent revealed for each of the tested preparations on 10, 14 and 21 st day. High antiviral activity of preparations in peak of an infection (10—14th day) is connected, with their immune modulating action.

From the infected mice the area of M-segment coding superficial glycoprotein G2 of hantavirus serotype Puumala was allocated and amplified. Certain nucleotide sequence of a gene was defined and cloned in the expression vector Pin-Point. ELISA reveals the certain antigenic properties of the recombinant protein.

Experience of teaching of Russian to the Chinese audience is stated. Importance of knowledge of lexicon and semantic features of investigated language and staging training is emphasized. At the initial stage of training semantic groups include neutral style and active lexicon. Further the passive lexicon, word-formation communications is studied, speech skills are improved. At all grade levels, irrespective of its character and the contents, it is necessary to base on lexicon and semantic system of Russian that allows learning speaking and hearing, reading and writing.

In this work some questions of the organization of scientific and techno parks — the major elements of modern market system, the organizational form of co-work of higher educational institutions, consulting and innovative firms, and the industrial enterprises are considered. As in Vladivostok there are all conditions for creation of the parks, the leading high schools of city could become the initiators in the decision of this problem that would promote increase in innovative potential of a high school science.

According to the purpose the system of introduction of results of research work in pedagogical activity of the faculty of medical high school has been developed. The structure of the system will consist of motivation, the purpose and problems, technological documents, forms and the contents of introduction, criteria of efficiency. The significant place in system is occupied by the structurization of a teaching material and designing on this basis of corresponding didactic units of mastering. The advanced pedagogical activity promotes increase of level of creative qualities, skills, and to development of the creative person of the future expert.